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Literature summary for 1.14.19.76 extracted from

  • Fliegmann, J.; Furtwaengler, K.; Malterer, G.; Cantarello, C.; Schueler, G.; Ebel, J.; Mithoefer, A.
    Flavone synthase II (CYP93B16) from soybean (Glycine max L.) (2010), Phytochemistry, 71, 508-514 .
    View publication on PubMed
Search for more literature for 1.14.19.76

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 eriodictyol pH 7.9, 25°C Glycine max
0.0025
-
 naringenin pH 7.9, 25°C Glycine max
0.0042
-
 liquiritigenin pH 7.9, 25°C Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Glycine max 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 Glycine max
-
 luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 Glycine max
-
 7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2 Glycine max
-
 apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max D3U591
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Glycine max luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 the enzymatic reaction of FNS II is stereoselective, favouring the (S)-enantiomer over the (R)-enantiomer Glycine max luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Glycine max 7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 the enzymatic reaction of FNS II is stereoselective, favouring the (S)-enantiomer over the (R)-enantiomer Glycine max 7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Glycine max apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2 the enzymatic reaction of FNS II is stereoselective, favouring the (S)-enantiomer over the (R)-enantiomer Glycine max apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP93B16
-
 Glycine max
FNS II
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Glycine max

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 heme-thiolate protein Glycine max

Expression

Organism Comment Expression
Glycine max induced by coronalon and by 12-oxo-phytodienoic acid. Jasmonic acid at a concentration of 0.1 mM is a comparatively weak inductor up

General Information

General Information Comment Organism
evolution phylogenetic analyses of the subfamily of plant CYP93B enzymes indicate the evolution of a gene encoding a flavone synthase which originally catalyzes the direct conversion of flavanones into flavones, via early gene duplication into a less efficient enzyme with an altered catalytic mechanism. Ultimately, this allows the evolution of the legume-specific isoflavonoid synthase activity Glycine max