Cloned (Comment) | Organism |
---|---|
gene CYP719A23, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis | Sinopodophyllum hexandrum |
gene CYP719A24, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis | Podophyllum peltatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state Michaelis-Menten kinetics | Sinopodophyllum hexandrum | |
additional information | - |
additional information | steady-state Michaelis-Menten kinetics | Podophyllum peltatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 | Sinopodophyllum hexandrum | - |
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 | Podophyllum peltatum | - |
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | Sinopodophyllum hexandrum | the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin | ? | - |
? | |
additional information | Podophyllum peltatum | the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Podophyllum peltatum | L7T720 | gene CYP719A24 | - |
Sinopodophyllum hexandrum | L7T8H2 | gene CYP719A23 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sinopodophyllum hexandrum | (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Podophyllum peltatum | (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 | (-)-matairesinol is isolated from Forsythia intermedia | Sinopodophyllum hexandrum | (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O | product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses | ? | |
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 | (-)-matairesinol is isolated from Forsythia intermedia | Podophyllum peltatum | (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O | product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses | ? | |
additional information | the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin | Sinopodophyllum hexandrum | ? | - |
? | |
additional information | the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin | Podophyllum peltatum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP719A23 | - |
Sinopodophyllum hexandrum |
CYP719A24 | - |
Podophyllum peltatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sinopodophyllum hexandrum |
25 | - |
assay at | Podophyllum peltatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sinopodophyllum hexandrum |
7.5 | - |
assay at | Podophyllum peltatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Sinopodophyllum hexandrum | |
cytochrome P450 | - |
Podophyllum peltatum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview | Sinopodophyllum hexandrum |
metabolism | the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview | Podophyllum peltatum |