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Literature summary for 1.14.19.53 extracted from

  • Johnson, K.; Phan, T.; Albertolle, M.; Peter Guengerich, F.
    Human mitochondrial cytochrome P450 27C1 is localized in skin and preferentially desaturates trans-retinol to 3,4-dehydroretinol (2017), J. Biol. Chem., 292, 13672-13687 .
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 all-trans-retinol pH 7.4, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens Q4G0S4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinol + 2 reduced adrenodoxin + 2 H+ + O2
-
 Homo sapiens all-trans-3,4-didehydroretinol + 2 oxidized adrenodoxin + 2 H2O
-
 ?
additional information kinetic isotope effects of 1.5–2.3 (on kcat/Km) are observed with 3,3-, 4,4-, and 3,3,4,4-deuterated retinol. Deuteration at C-4 produces a 4fold increase in 3-hydroxylation due to metabolic switching, with no observable effect on 4-hydroxylation. Deuteration at C-3 produces a strong kinetic isotope effect for 3-hydroxylation but not 4-hydroxylation Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.068
-
 all-trans-retinol pH 7.4, 37°C Homo sapiens

General Information

General Information Comment Organism
physiological function the most likely catalytic mechanism begins with abstraction of a hydrogen atom from C-4 (or possibly C-3) initiating the desaturation pathway, followed by a sequential abstraction of a hydrogen atom or proton-coupled electron transfer. Adrenodoxin reduction and hydrogen abstraction both contribute to rate limitation Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
61
-
 all-trans-retinol pH 7.4, 37°C Homo sapiens