Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Coptis japonica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | sequence contains a putative endoplasmic reticulum-localizing signal | Coptis japonica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | Coptis japonica | - |
(S)-corytuberine + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coptis japonica | A8CDR5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Coptis japonica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-codamine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Coptis japonica | 2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O | 12% of the activity with reticuline. The major product is (R,S)-orientaline, 4'-O-demethylation product of (R,S)-codamine, and the minor one is proposed to be the same compound as (R,S)-orientaline-derived phenol coupling product | ? | |
(R,S)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2 | - |
Coptis japonica | 2,10-dimethoxy-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,11-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O | 19% of the activity with reticuline | ? | |
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Coptis japonica | 2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O | 2% of the activity with reticuline | ? | |
(S)-orientaline + [reduced NADPH-hemoprotein reductase] + O2 | - |
Coptis japonica | 2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O | 2.7% of the activity with reticuline | ? | |
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | - |
Coptis japonica | (S)-corytuberine + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
additional information | CĀC phenol-coupling reaction, requires NADPH and oxygen. The enzyme is specific for S-enantiomer of substrate | Coptis japonica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
corytuberine synthase | - |
Coptis japonica |
CYP80G2 | - |
Coptis japonica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Coptis japonica |