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Literature summary for 1.14.19.48 extracted from

  • Schuster, J.; Schaefer, F.; Huebler, N.; Brandt, A.; Rosell, M.; Haertig, C.; Harms, H.; Mueller, R.H.; Rohwerder, T.
    Bacterial degradation of tert-amyl alcohol proceeds via hemiterpene 2-methyl-3-buten-2-ol by employing the tertiary alcohol desaturase function of the Rieske nonheme mononuclear iron oxygenase MdpJ (2012), J. Bacteriol., 194, 972-981.
    View publication on PubMedView publication on EuropePMC
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Organism

Organism UniProt Comment Textmining
Aquincola tertiaricarbonis G8FRC5
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-
Aquincola tertiaricarbonis L108 G8FRC5
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-3-pentanol + NADPH + H+ + O2
-
 Aquincola tertiaricarbonis 3-methyl-1-penten-3-ol + NADP+ + 2 H2O
-
 ?
3-methyl-3-pentanol + NADPH + H+ + O2
-
 Aquincola tertiaricarbonis L108 3-methyl-1-penten-3-ol + NADP+ + 2 H2O
-
 ?
additional information MdpJ is not hydroxylating tert-amyl alcohol to diols but functions as a desaturase, resulting in the formation of the hemiterpene 3-methyl-1-buten-3-ol. The latter is further processed via the hemiterpenes prenol, prenal, and 3-methylcrotonic acid Aquincola tertiaricarbonis ?
-
 ?
additional information MdpJ is not hydroxylating tert-amyl alcohol to diols but functions as a desaturase, resulting in the formation of the hemiterpene 3-methyl-1-buten-3-ol. The latter is further processed via the hemiterpenes prenol, prenal, and 3-methylcrotonic acid Aquincola tertiaricarbonis L108 ?
-
 ?
tert-amyl alcohol + NADPH + H+ + O2
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 Aquincola tertiaricarbonis isoprenyl alcohol + NADP+ + 2 H2O
-
 ?
tert-amyl alcohol + NADPH + H+ + O2
-
 Aquincola tertiaricarbonis L108 isoprenyl alcohol + NADP+ + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
MdpJ
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 Aquincola tertiaricarbonis

General Information

General Information Comment Organism
physiological function MdpJ knockout mutants are not able to grow on the tertiary alcohols tert-butanol and tert-amyl alcohol, whereas 2-methylpropan-1,2-diol, the putative product of tert-butanol hydroxylation by MdpJ catalysis can still be used as the sole source of energy and carbon. The postulated 2-methylpropan-1,2-diol intermediate 2-hydroxyisobutanoate is metabolized by wild-type and mutant strains at the same rates. tert-amyl alcohol and hemiterpene degradation are linked with the catabolic route of leucine Aquincola tertiaricarbonis