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Literature summary for 1.14.19.47 extracted from

  • Zhou, X.-R.; Robert S.; Singh, S.; Green, A.
    Heterologous production of GLA and SDA by expression of an Echium plantagineum DELTA6-desaturase gene (2007), Plant Sci., 172, 421-422.
No PubMed abstract available
Search for more literature for 1.14.19.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana tabacum leaf and Arabidopsis thaliana seed Echium plantagineum

Protein Variants

Protein Variants Comment Organism
D169V/T275A the mutant has little effect on the D6-desaturation activity of the enzyme Echium plantagineum
L14P the mutant shows dramatically reduced activity compared to the wild type enzyme Echium plantagineum
L224S the mutant shows dramatically reduced activity compared to the wild type enzyme Echium plantagineum
L371F the mutant shows dramatically reduced activity compared to the wild type enzyme Echium plantagineum
S205N the mutant has little effect on the D6-desaturation activity of the enzyme Echium plantagineum
S301P the mutant shows dramatically reduced activity compared to the wild type enzyme Echium plantagineum
S391P the mutant shows dramatically reduced activity compared to the wild type enzyme Echium plantagineum
V137A the mutant shows dramatically reduced activity compared to the wild type enzyme Echium plantagineum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Echium plantagineum
-
 stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Echium plantagineum preferred substrate gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Echium plantagineum Q2M488
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Echium plantagineum stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ preferred substrate Echium plantagineum gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
D6Des
-
 Echium plantagineum
DELTA6-desaturase
-
 Echium plantagineum

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
 Echium plantagineum