BRENDA - Enzyme Database show
show all sequences of 1.14.19.47

Isolation of a DELTA6-desaturase gene from the cyanobacterium Synechocystis sp. strain PCC 6803 by gain-of-function expression in Anabaena sp.strain PCC 7120

Reddy, A.S.; Nuccio, M.L.; Gross, L.M.; Thomas, T.L.; Plant Mol. Biol. 22, 293-300 (1993)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Anabaena sp.
Synechocystis sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Synechocystis sp.
-
stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Synechocystis sp.
-
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechocystis sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734925
Synechocystis sp.
stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734925
Synechocystis sp.
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Synechocystis sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Anabaena sp.
Synechocystis sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Synechocystis sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Synechocystis sp.
-
stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Synechocystis sp.
-
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734925
Synechocystis sp.
stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734925
Synechocystis sp.
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
Other publictions for EC 1.14.19.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744748
Nayak
Dietary fish oil replacement ...
Barbonymus gonionotus
Comp. Biochem. Physiol. B
205
1-12
2017
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
744592
Jeennor
The codon-optimized {incremen ...
Pythium sp., Pythium sp. BCC53698
BMC Biotechnol.
15
82
2015
-
2
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
744947
Wang
Transgenic expression of salm ...
Salmo salar
Fish Shellfish Immunol.
39
223-230
2014
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746258
Xu
Regulation of tissue LC-PUFA ...
Lateolabrax japonicus
PLoS ONE
9
e87726
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
2
-
-
746261
Xie
Cloning, functional character ...
Scatophagus argus
PLoS ONE
9
e90200
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
726857
Chen
Delta-6 desaturase from borage ...
Borago officinalis
Biochem. Biophys. Res. Commun.
410
484-488
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734976
Zhou
-
Heterologous production of GLA ...
Echium plantagineum
Plant Sci.
172
421-422
2007
-
-
1
-
8
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734928
Kajikawa
Isolation and characterization ...
Marchantia polymorpha
Plant Mol. Biol.
54
335-352
2004
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734988
Whitney
Functional characterisation of ...
Anemone leveillei
Planta
217
983-992
2003
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734669
Cook
Modification of fatty acid com ...
Borago officinalis
Mol. Biotechnol.
21
123-128
2002
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733244
Laoteng
DELTA6-Desaturase of Mucor rou ...
Amylomyces rouxii, Amylomyces rouxii ATCC 24905
Biochem. Biophys. Res. Commun.
279
17-22
2000
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733927
Sakuradani
DELTA6-fatty acid desaturase f ...
Mortierella alpina, Mortierella alpina 1S-4
Gene
238
445-453
1999
-
-
1
-
-
-
-
-
-
-
1
4
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734601
Huang
Cloning of DELAT12- and DELTA6 ...
Mortierella alpina
Lipids
34
649-659
1999
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734946
Sayanova
Histidine-41 of the cytochrome ...
Borago officinalis
Plant Physiol.
121
641-646
1999
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733370
Marzo
Biosynthesis of docosahexaenoi ...
Homo sapiens
Biochim. Biophys. Acta
1301
263-272
1996
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
734532
Biagi
The effect of dietary polyenyl ...
Rattus norvegicus
J. Nutr. Biochem.
4
690-694
1993
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
734925
Reddy
Isolation of a DELTA6-desatura ...
Synechocystis sp.
Plant Mol. Biol.
22
293-300
1993
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-