Cloned (Comment) | Organism |
---|---|
gene CsFAD2, variant FAD2-1, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview | Camelina sativa |
gene CsFAD2, variant FAD2-2, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview | Camelina sativa |
gene CsFAD2, variant FAD2-3, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A . The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview | Camelina sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Camelina sativa | 5783 | - |
membrane | integral membrane enzyme | Camelina sativa | 16020 | - |
microsome | - |
Camelina sativa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | two Fe2+ ions bound in the His box | Camelina sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelina sativa | E7CCB4 | FAD2-2 | - |
Camelina sativa | E7CCB6 | FAD2-1 | - |
Camelina sativa | M4HN37 | FAD2-3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Camelina sativa | - |
hypocotyl | - |
Camelina sativa | - |
leaf | - |
Camelina sativa | - |
root | - |
Camelina sativa | - |
seed | high expression level, particularly from 6 to 12 DAF and from 18 to 24 DAF,corresponding with the phases of seed expansion and oil accumula-tion respectively | Camelina sativa | - |
seed | high expression level, particularly from 6 to 12 days after flowering and from 18 to 24 days after flowering , corresponding with the phases of seed expansion and oil accumulation respectively | Camelina sativa | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 44100, about, sequence calculation | Camelina sativa |
Synonyms | Comment | Organism |
---|---|---|
CsFAD2 | - |
Camelina sativa |
FAD2 | - |
Camelina sativa |
FAD2-1 | - |
Camelina sativa |
FAD2-2 | - |
Camelina sativa |
FAD2-3 | - |
Camelina sativa |
oleate DELTA12desaturase | - |
Camelina sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Camelina sativa | sequence calculation | - |
8 |
Camelina sativa | sequence calculation | - |
8.1 |
General Information | Comment | Organism |
---|---|---|
evolution | three different copies of the genes FAD2 and FAD3 are identified, which contain three histidine rich motifs (HXCGHX, HRXHH andHVXHH) and six highly conserved transmembrane domains. Comparing their sequences, the CsFAD2copies accommodate four conservative changes (E36D, R48H, V97A, and A177P) and two semi-conservative ones (V63I and L249M), whereas only one semi-conservative change (A327S) is detected in CsFAD3 but with two extra amino acids (H147 and G148) | Camelina sativa |