BRENDA - Enzyme Database show
show all sequences of 1.14.19.41

Overexpression of CYP710A1 and CYP710A4 in transgenic Arabidopsis plants increases the level of stigmasterol at the expense of sitosterol

Arnqvist, L.; Persson, M.; Jonsson, L.; Dutta, P.C.; Sitbon, F.; Planta 227, 309-317 (2008)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
24-methylcholesterol + NADPH + H+ + O2
Arabidopsis thaliana
-
brassicasterol + NADP+ + H2O
-
-
?
beta-sitosterol + NADPH + H+ + O2
Arabidopsis thaliana
-
stigmasterol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O64697
-
-
Arabidopsis thaliana
Q9ZV28
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
24-methylcholesterol + NADPH + H+ + O2
-
734989
Arabidopsis thaliana
brassicasterol + NADP+ + H2O
-
-
-
?
beta-sitosterol + NADPH + H+ + O2
-
734989
Arabidopsis thaliana
stigmasterol + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
;
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
24-methylcholesterol + NADPH + H+ + O2
Arabidopsis thaliana
-
brassicasterol + NADP+ + H2O
-
-
?
beta-sitosterol + NADPH + H+ + O2
Arabidopsis thaliana
-
stigmasterol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
24-methylcholesterol + NADPH + H+ + O2
-
734989
Arabidopsis thaliana
brassicasterol + NADP+ + H2O
-
-
-
?
beta-sitosterol + NADPH + H+ + O2
-
734989
Arabidopsis thaliana
stigmasterol + NADP+ + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme functions in stigmasterol synthesis; the enzyme functions in stigmasterol synthesis
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme functions in stigmasterol synthesis
Arabidopsis thaliana
Other publictions for EC 1.14.19.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745006
Zhang
De novo sequencing and transc ...
Periploca sepium, Periploca sepium Bunge
Front. Plant sci.
8
594
2017
-
-
-
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5
-
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-
744282
Valitova
Plant Sterols Diversity, Bios ...
Saccharomyces cerevisiae
Biochemistry (Moscow)
81
819-834
2016
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-
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2
-
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1
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1
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2
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1
-
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1
1
-
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746505
Mueller
Fungal sterol C22-desaturase ...
Candida glabrata, Candida glabrata NCCLS 84, Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 9763, Yarrowia lipolytica, Yarrowia lipolytica UCDFST 60-26
Steroids
101
1-6
2015
-
-
-
-
-
-
3
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9
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-
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-
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-
-
-
-
-
-
-
-
1
1
-
-
-
733910
Sun
Sterol C-22 desaturase ERG5 me ...
Fusarium verticillioides, Neurospora crassa
Front. Microbiol.
4
127
2013
-
-
-
-
-
-
6
-
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-
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-
4
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2
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2
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2
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6
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2
-
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-
-
-
-
-
1
1
-
-
-
733106
Martel
A clinical isolate of Candida ...
Candida albicans
Antimicrob. Agents Chemother.
54
3578-3583
2010
-
-
-
-
1
-
5
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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-
-
1
-
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-
-
1
-
1
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-
5
-
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-
1
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-
-
2
-
-
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-
-
-
-
-
-
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-
-
-
-
-
734990
Morikawa
CYP710A genes encoding sterol ...
Physcomitrella patens
Planta
229
1311-1322
2009
-
-
1
-
-
-
-
2
1
-
2
1
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
1
-
2
1
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
734014
Whitaker
Ripening-specific stigmasterol ...
Solanum lycopersicum
J. Agric. Food Chem.
56
3828-3835
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
4
-
-
1
-
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-
-
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-
-
1
-
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-
-
1
-
-
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-
-
-
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-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
734989
Arnqvist
Overexpression of CYP710A1 and ...
Arabidopsis thaliana
Planta
227
309-317
2008
-
-
-
-
-
-
-
-
-
-
-
4
-
6
-
-
-
-
-
-
-
-
4
-
-
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-
-
-
-
-
1
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-
2
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-
4
-
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-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
733311
Morikawa
Cytochrome P450 subfamily CYP7 ...
Arabidopsis thaliana
Biochem. Soc. Trans.
34
1202-1205
2006
-
-
-
-
-
-
-
2
-
-
-
2
-
5
-
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6
-
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-
2
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-
1
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2
-
-
-
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-
-
2
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-
2
-
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-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
734886
Morikawa
Cytochrome P450 CYP710A encode ...
Arabidopsis thaliana, Solanum lycopersicum
Plant Cell
18
1008-1022
2006
-
-
2
-
-
-
-
2
2
-
4
-
-
7
-
-
-
-
-
-
-
-
5
2
-
-
-
2
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
2
2
-
4
-
-
-
-
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-
-
-
-
5
2
-
-
-
2
-
-
-
-
-
-
-
-
2
2
733104
Lamb
Purification, reconstitution, ...
Candida glabrata, Candida glabrata L5DU61
Antimicrob. Agents Chemother.
43
1725-1728
1999
-
-
-
-
-
-
3
1
1
-
1
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-
6
-
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1
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2
1
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1
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3
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1
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3
3
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1
1
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1
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1
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2
1
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-
733848
Kelly
Sterol 22-desaturase, cytochro ...
Saccharomyces cerevisiae
FEBS Lett.
412
233-235
1997
-
-
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2
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2
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1
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1
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1
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2
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1
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-
734125
Kelly S.L
Characterization of Saccharomy ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae DK2
J. Biol. Chem.
272
9986-9988
1997
-
-
-
-
-
-
2
1
2
-
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-
4
-
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1
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2
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1
1
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2
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1
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2
2
1
1
2
-
-
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1
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-
2
-
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-
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-
-
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-
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-
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-
733926
Skaggs
Cloning and characterization o ...
Saccharomyces cerevisiae
Gene
169
105-109
1996
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
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1
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1
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1
1
-
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-
733847
Kelly
Purification and reconstitutio ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae DK2
FEBS Lett.
377
217-220
1995
-
-
-
-
-
-
1
-
1
-
1
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-
5
-
-
1
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-
-
2
1
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1
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1
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1
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1
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1
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1
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2
1
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-
733242
Giner
Inhibition and substrate speci ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
173
60-66
1990
-
-
-
-
-
-
2
-
1
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1
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3
-
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1
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1
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1
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1
2
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1
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3
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