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Literature summary for 1.14.19.41 extracted from
- Purification, reconstitution, and inhibition of cytochrome P-450 sterol DELTA22-desaturase from the pathogenic fungus Candida glabrata (1999), Antimicrob. Agents Chemother., 43, 1725-1728.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fluconazole | competitive inhibition | [Candida] glabrata |  |
| itraconazole | - |
[Candida] glabrata | |
| ketoconazole | - |
[Candida] glabrata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0125 | - |
ergosta-5,7-dienol | at pH 6.8 and 37°C | [Candida] glabrata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
[Candida] glabrata | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
x * 59000, SDS-PAGE | [Candida] glabrata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] glabrata | - |
- |
- |
| [Candida] glabrata L5DU61 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amino-octyl Sepharose column chromatography and hydroxyapatite column chromatography | [Candida] glabrata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ergosta-5,7-dienol + NADPH + H+ + O2 | - |
[Candida] glabrata | ergosterol + NADP+ + H2O | - |
? | |
| ergosta-5,7-dienol + NADPH + H+ + O2 | - |
[Candida] glabrata L5DU61 | ergosterol + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59000, SDS-PAGE | [Candida] glabrata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytochrome P-450 sterol DELTA22-desaturase | - |
[Candida] glabrata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
[Candida] glabrata | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000001 | - |
calculated value, pH and temperature not specified in the publication | [Candida] glabrata | ketoconazole | |
| 0.0000001 | - |
calculated value, pH and temperature not specified in the publication | [Candida] glabrata | fluconazole | |
| 0.0000001 | - |
calculated value, pH and temperature not specified in the publication | [Candida] glabrata | itraconazole | |
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