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Literature summary for 1.14.19.35 extracted from

  • Matsuda, O.; Sakamoto, H.; Hashimoto, T.; Iba, K.
    A temperature-sensitive mechanism that regulates post-translational stability of a plastidial omega-3 fatty acid desaturase (FAD8) in Arabidopsis leaf tissues (2005), J. Biol. Chem., 280, 3597-3604.
    View publication on PubMed
Search for more literature for 1.14.19.35

Cloned(Commentary)

Cloned (Comment) Organism
gene fad7, expression of wild-type and chimeric mutant enzymes with or without a c-Myc epitope tag in transgenic Arabidopsis thaliana plants Arabidopsis thaliana
gene fad8, expression of wild-type and chimeric mutant enzymes with or without a c-Myc epitope tag in transgenic Arabidopsis thaliana plants Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of a fad7/fad8 double mutant with reduced overall levels of trienoic fatty acids in leaf tissues. A series of FAD7-FAD8 chimeric genes, each encoding a functional plastidial omega-3 desaturase, are introduced into the Arabidopsis thaliana fad7/fad8 double mutant, structures, overview. All transformants show an unaltered temperature response, and none of the transformants exhibit an exceptional phenotype Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information elevated temperatures lead to decreases in leaf trienoic fatty acid level due to temperature sensitivity of enzyme FAD8 activity Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P46310 gene fad7
-
Arabidopsis thaliana P48622 gene FAD8
-
Arabidopsis thaliana Col-0 P46310 gene fad7
-
Arabidopsis thaliana Col-0 P48622 gene FAD8
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Synonyms

Synonyms Comment Organism
FAD7
-
 Arabidopsis thaliana
FAD8
-
 Arabidopsis thaliana
omega-3 desaturase
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 elevated temperatures lead to decreases in leaf trienoic fatty acid level due to temperature sensitivity of enzyme FAD8 activity Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana temperature-sensitive FAD8 expression additional information

General Information

General Information Comment Organism
additional information elevated temperatures lead to decreases in leaf trienoic fatty acid level due to temperature sensitivity of enzyme FAD8 activity, the C-terminal region of FAD8 desaturase is essential for destabilization at high temperature (22°C). The C-terminal coding region of FAD8 is sufficient to suppress the accumulation of plastidial omega-3 desaturase protein at high temperature, overview Arabidopsis thaliana