Cloned (Comment) | Organism |
---|---|
gene DsFAD2-1, DNA and amino acid sequence determination and analysis, recombinant expression of DsFAD2-1 in Saccharomyces cerevisiae strain YPH499 and Glycine max somatic embryos resulting in the accumulation of the trans-DELTA12 isomer of linoleic acid (18:29cis,12trans) rather than the more typical cis-DELTA12 isomer. When co-expressed with DsFAD2-1 in soybean embryos or yeast, DsFAD2-2 converts 18:29cis,12trans into dimorphecolic acid | Dimorphotheca sinuata |
gene DsFAD2-2, DNA and amino acid sequence determination and analysis, recombinant expression of DsFAD2-2 in Saccharomyces cerevisiae strain YPH499 and Glycine max somatic embryos. When co-expressed with DsFAD2-1 in soybean embryos or yeast, DsFAD2-2 converts 18:29cis,12trans into dimorphecolic acid. When DsFAD2-2 is expressed alone in soybean embryos or together with a typical cis-DELTA12-oleic acid desaturase in yeast, trace amounts of the cis-DELTA12 isomer of dimorphecolic acid (9-OH-18:2DELTA9cis,12cis) are formed from DsFAD2-2 activity with cis-DELTA12-linoleic acid | Dimorphotheca sinuata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | diiron-oxo center | Dimorphotheca sinuata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | Dimorphotheca sinuata | - |
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
additional information | Dimorphotheca sinuata | seeds of Dimorphotheca species fatty acids contains structural features that are not typically found in plant fatty acids, including a C-9 hydroxyl group, DELTA10,DELTA12-conjugated double bonds, and trans-DELTA12 unsaturation | ? | - |
? | |
additional information | Dimorphotheca sinuata | seeds of Dimorphotheca species fatty acids contains structural features that are not typically found in plant fatty acids, including a C-9 hydroxyl group, DELTA10,DelTA12-conjugated double bonds, and trans-DELTA12 unsaturation. | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dimorphotheca sinuata | Q6RS95 | gene DsFAD2-1 | - |
Dimorphotheca sinuata | Q6RS96 | gene DsFAD2-2 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | proposed mechanism of DsFAD2-2: removal of a C-11 hydrogen atom from trans-DELTA12-linoleic acid by the diiron-oxo center of DsFAD2-2 results in the shift of the cis-DELTA9 double bond to the trans-DELTA10 position. The radical remaining at the C-9 position is then able to remove an oxygen atom from the diiron-oxo center because of close proximity to the catalytic core of DsFAD2-2 as dictated by the substrate binding properties of this enzyme | Dimorphotheca sinuata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Dimorphotheca sinuata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Dimorphotheca sinuata | a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
additional information | seeds of Dimorphotheca species fatty acids contains structural features that are not typically found in plant fatty acids, including a C-9 hydroxyl group, DELTA10,DELTA12-conjugated double bonds, and trans-DELTA12 unsaturation | Dimorphotheca sinuata | ? | - |
? | |
additional information | seeds of Dimorphotheca species fatty acids contains structural features that are not typically found in plant fatty acids, including a C-9 hydroxyl group, DELTA10,DelTA12-conjugated double bonds, and trans-DELTA12 unsaturation. | Dimorphotheca sinuata | ? | - |
? | |
additional information | isozyme DsFAD2-2 catalyzes the conversion of the DELTA9 double bond of linoleic acid into a C-9 hydroxyl group and DELTA10trans double bond and displays a substrate preference for the trans-DELTA12, rather than the cis-DELTA12, isomer of linoleic acid | Dimorphotheca sinuata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA12-oleic acid desaturase | - |
Dimorphotheca sinuata |
DsFAD2-1 | - |
Dimorphotheca sinuata |
DsFAD2-2 | - |
Dimorphotheca sinuata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Dimorphotheca sinuata |
General Information | Comment | Organism |
---|---|---|
evolution | the evolution of two divergent DELTA12-oleic acid desaturases for the biosynthesis of an unusual fatty acid | Dimorphotheca sinuata |
metabolism | biosynthetic pathway of dimorphecolic acid involving the concerted activities of DsFAD2-1 and DsFAD2-2 | Dimorphotheca sinuata |
physiological function | involvement of DsFAD2-1 in dimorphecolic acid synthesis | Dimorphotheca sinuata |
physiological function | involvement of DsFAD2-2 in dimorphecolic acid synthesis | Dimorphotheca sinuata |