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Literature summary for 1.14.19.31 extracted from

  • Qiu, X.; Hong, H.; MacKenzie, S.L.
    Identification of a DELTA4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea (2001), J. Biol. Chem., 276, 31561-31566.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Saccharomyces cerevisiae and Brassica juncea respectively. Introduction of the full-length enzyme into Brassica juncea under the control of the 35S promoter results in the production of (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaeno in leaves when exogenously supplied with (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoic acid Thraustochytrium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49800
-
x * 49800, calculated fropm sequence Thraustochytrium sp.

Organism

Organism UniProt Comment Textmining
Thraustochytrium sp. Q8S3C0
-
-
Thraustochytrium sp. ATCC 21685 Q8S3C0
-
-

Reaction

Reaction Comment Organism Reaction ID
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O (1) Thraustochytrium sp.
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O (1) Thraustochytrium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
Thraustochytrium sp. a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
?
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
Thraustochytrium sp. ATCC 21685 a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
?
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
Thraustochytrium sp. a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
?
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
Thraustochytrium sp. ATCC 21685 a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
?

Subunits

Subunits Comment Organism
? x * 49800, calculated fropm sequence Thraustochytrium sp.

Synonyms

Synonyms Comment Organism
DELTA4 fatty acid desaturase
-
Thraustochytrium sp.

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of docosahexanoic acid Thraustochytrium sp.