Cloned (Comment) | Organism |
---|---|
expression of wild-type, A181T/A188G/Y189F/S205N/L206T/G207A, A188G/Y189F, A181T/A200F and A181T/A200F/S205N/L206T/G207A mutant enzyme in Escherichia coli | Thunbergia alata |
Protein Variants | Comment | Organism |
---|---|---|
A181T/A188G/Y189F/S205N/L206T/G207A | compared to wild-type: reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate, exhibits DELTA9 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
A181T/A200F | increase in DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
A181T/A200F/S205N/L206T/G207A | reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, very low DELTA9 desaturase activity, no DELTA6 desaturase activity with stearoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
A188G/Y189F | reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thunbergia alata | - |
black-eyed Susan vine | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thunbergia alata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
substrate palmitoyl-[acyl-carrier protein] | Thunbergia alata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Thunbergia alata | 6-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Thunbergia alata | 6-octadecenoyl-[acyl-carrier protein] + 9-octadecenoyl-[acyl-carrier protein]acceptor + H2O | ratio of activity: 2/1 | ? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Thunbergia alata |