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Literature summary for 1.14.19.3 extracted from

  • Cahoon, E.B.; Lindqvist, Y.; Schneider, G.; Shanklin, J.
    Redesign of soluble fatty acid desaturases from plants from altered substrate specificity and double bond position (1997), Proc. Natl. Acad. Sci. USA, 94, 4872-4877.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, A181T/A188G/Y189F/S205N/L206T/G207A, A188G/Y189F, A181T/A200F and A181T/A200F/S205N/L206T/G207A mutant enzyme in Escherichia coli Thunbergia alata

Protein Variants

Protein Variants Comment Organism
A181T/A188G/Y189F/S205N/L206T/G207A compared to wild-type: reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate, exhibits DELTA9 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate Thunbergia alata
A181T/A200F increase in DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate Thunbergia alata
A181T/A200F/S205N/L206T/G207A reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, very low DELTA9 desaturase activity, no DELTA6 desaturase activity with stearoyl-[acyl-carrier protein] as substrate Thunbergia alata
A188G/Y189F reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate Thunbergia alata

Organism

Organism UniProt Comment Textmining
Thunbergia alata
-
black-eyed Susan vine
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thunbergia alata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
substrate palmitoyl-[acyl-carrier protein] Thunbergia alata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
-
Thunbergia alata 6-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
-
Thunbergia alata 6-octadecenoyl-[acyl-carrier protein] + 9-octadecenoyl-[acyl-carrier protein]acceptor + H2O ratio of activity: 2/1 ?

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
Thunbergia alata