Cloned (Comment) | Organism |
---|---|
gene PtD8A, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1 | Populus tomentosa |
gene PtD8B, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1 | Populus tomentosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | Populus tomentosa | - |
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | Populus tomentosa | - |
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
additional information | Populus tomentosa | the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tomentosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant functional enzyme from Saccharomyces cerevisiae strain INVSc1 | Populus tomentosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Populus tomentosa | - |
leaf | higher expression level | Populus tomentosa | - |
additional information | PtD8A is expressed in all tissues analyzed. Expression level of PtD8C is clearly much lower than that of PtD8A and PtD8B | Populus tomentosa | - |
additional information | PtD8B is expressed in all tissues analyzed. Expression level of PtD8C is clearly much lower than that of PtD8A and PtD8B | Populus tomentosa | - |
additional information | PtD8C is not expressed in roots. Expression level of PtD8C is clearly much lower than that of PtD8A and PtD8B | Populus tomentosa | - |
root | - |
Populus tomentosa | - |
stem | - |
Populus tomentosa | - |
stem | higher expression level | Populus tomentosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Populus tomentosa | a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Populus tomentosa | a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
additional information | the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine | Populus tomentosa | ? | - |
? | |
additional information | isozyme PtD8A preferably forms (8E)-products, ratio 8(Z):8(E) is 0.25 | Populus tomentosa | ? | - |
? | |
additional information | isozyme PtD8B preferably forms (8E)-products, ratio 8(Z):8(E) is 0.17 | Populus tomentosa | ? | - |
? | |
additional information | isozyme PtD8C preferably forms (8Z)-products, ratio 8(Z):8(E) is 12.71 | Populus tomentosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA8-sphingolipid desaturase | - |
Populus tomentosa |
PtD8A | - |
Populus tomentosa |
PtD8B | - |
Populus tomentosa |
PtD8C | - |
Populus tomentosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Populus tomentosa |
General Information | Comment | Organism |
---|---|---|
physiological function | the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants | Populus tomentosa |