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Literature summary for 1.14.19.28 extracted from

  • Garba, L.; Mohamad Ali, M.S.; Oslan, S.N.; Rahman, R.N.
    Molecular cloning and functional expression of a DELTA9-fatty acid desaturase from an antarctic Pseudomonas sp. A3 (2016), PLoS ONE, 11, e0160681 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.28

Cloned(Commentary)

Cloned (Comment) Organism
gene PA3FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli, GCMS analysis reveals that palmitoleic (C16:1DELTA9) and oleic acids (C18:1DELTA9) are the only unsaturated fatty acids identified in addition to the endogenous fatty acids, expression at 15°C Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane according to transmembrane helices prediction, the protein has two hydrophobic domains located between amino acids 10-32 and 135-157 and thought to be long enough to span the membrane bilayer two times with the two protein termini facing the cytosol Pseudomonas sp. 16020
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additional information sequence analysis reveals a potential N-terminal signal peptide Pseudomonas sp.
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp.
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 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp. A3(2015c)
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 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp.
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 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp. A3(2015c)
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 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. A0A0S1Z203
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Pseudomonas sp. A3(2015c) A0A0S1Z203
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. A3(2015c) 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. A3(2015c) 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, about, sequence calculation Pseudomonas sp.

Synonyms

Synonyms Comment Organism
DELTA9-fatty acid desaturase
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 Pseudomonas sp.
DELTA9-fatty acid desaturase-like protein
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 Pseudomonas sp.
More cf. EC 1.14.19.27 Pseudomonas sp.
PA3FAD9
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 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. sequence calculation
-
 9.48