BRENDA - Enzyme Database
show all sequences of 1.14.19.28

Heterologous expression of PA8FAD9 and functional characterization of a DELTA9-fatty acid desaturase from a cold-tolerant Pseudomonas sp. A8

Garba, L.; Ali, M.S.; Oslan, S.N.; Rahman, R.N.; Mol. Biotechnol. 58, 718-728 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein
Pseudomonas sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A8(2016b)
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
A0A165DNG6
-
-
Pseudomonas sp. A8(2016b)
A0A165DNG6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp.
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp. A8(2016b)
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 45000, about, sequence calculation
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Pseudomonas sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A8(2016b)
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp.
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp. A8(2016b)
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, about, sequence calculation
Pseudomonas sp.
General Information
General Information
Commentary
Organism
physiological function
the D9-fatty acid desaturase initiates the synthesis of polyunsaturated fatty acids by introducing the first double bond at the C9 position of saturated fatty acid chains. These substrates can further be elongated by other classes of desaturase enzymes
Pseudomonas sp.
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the D9-fatty acid desaturase initiates the synthesis of polyunsaturated fatty acids by introducing the first double bond at the C9 position of saturated fatty acid chains. These substrates can further be elongated by other classes of desaturase enzymes
Pseudomonas sp.
Other publictions for EC 1.14.19.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744938
Sinetova
Polyphasic characterization o ...
Desertifilum sp. IPPAS B-1220
FEMS Microbiol. Lett.
364
doi: 10.1093/femsle/fnx027
2017
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745715
Woelke
DELTA9 desaturase from Trypan ...
Trypanosoma cruzi, Trypanosoma cruzi Tulahuen
Microbiol. Res.
194
29-37
2017
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2
2
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745749
Garba
Heterologous expression of PA ...
Pseudomonas sp., Pseudomonas sp. A8(2016b)
Mol. Biotechnol.
58
718-728
2016
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746210
Garba
Molecular cloning and functio ...
Pseudomonas sp., Pseudomonas sp. A3(2015c)
PLoS ONE
11
e0160681
2016
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746429
Gerasymenko
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Characterization of Nicotiana ...
Thermosynechococcus vulcanus
Russ. J. Plant Physiol.
62
283-291
2015
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735247
Popov
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Chloroplast ultrastructure in ...
Thermosynechococcus vulcanus
Russ. J. Plant Physiol.
54
278-281
2007
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735246
Popov
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Specific features of oxidative ...
Thermosynechococcus vulcanus
Russ. J. Plant Physiol.
53
469-473
2006
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735245
Popov
-
The effect of tobacco plant tr ...
Thermosynechococcus vulcanus
Russ. J. Plant Physiol.
52
664-667
2005
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733677
Meesapyodsuk
Probing the mechanism of a cya ...
Arthrospira platensis, Arthrospira platensis C1
Comp. Biochem. Physiol. B
129
831-835
2001
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734509
Kiseleva
Expression of the gene for the ...
Thermosynechococcus vulcanus
J. Mol. Microbiol. Biotechnol.
2
331-338
2000
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734880
Fukuchi-Mizutani
Characterization of DELTA9 acy ...
Arabidopsis thaliana
Plant Cell Physiol.
39
247-253
1998
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733882
Sakamoto
Low-temperature-induced desatu ...
Synechococcus sp.
FEMS Microbiol. Lett.
152
313-320
1997
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732021
Sakamoto
DELTA9 Acyl-lipid desaturases ...
Synechocystis sp., Trichormus variabilis
J. Biol. Chem.
269
25576-25580
1994
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732646
Higashi
An in vivo study of substrate ...
Synechocystis sp.
Plant Physiol.
102
1275-1278
1993
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