Cloned (Comment) | Organism |
---|---|
DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis | Penaeus monodon |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction and expression of double-stranded RNA specific to PmNvd (dsNvd) for silencing of the enzyme, effect of PmNvd knockdown on molting duration, phenotype, overview | Penaeus monodon |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme sequence contains the transmembrane motif 33WxxF59 | Penaeus monodon | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H | Penaeus monodon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus monodon | A0A2I4PEL8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the expression of PmNvd is dominantly in the Y-organ, but changes during molting period. The PmNvd mRNA level is low in intermolt and early premolt stages, then dramatically increases in the mid premolt stage. No enzyme expression in eyestalk, brain, thoracic ganglia, nerve cord, hepatopancreas, ovary, gills, muscle, and lymphoid organ, RT-PCR expression analysis | Penaeus monodon | - |
Y-organ | exclusive expression | Penaeus monodon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 + NAD(P)H + H+ | - |
Penaeus monodon | cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholesterol 7,8-dehydrogenase | - |
Penaeus monodon |
PmNvd | - |
Penaeus monodon |
Rieske domain oxygenase Neverland | - |
Penaeus monodon |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[2Fe-2S]-center | the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H | Penaeus monodon |
General Information | Comment | Organism |
---|---|---|
malfunction | silencing of PmNvd transcript in shrimp by specific double-stranded RNA (dsNvd) leads to prolonged molt duration with abnormal molting progression, i.e. the molting process got stuck at early premolt stage. In addition, the 20-hydroxyecdysone titer in hemolymph of dsNvd-injected shrimps is significantly reduced compared with that in NaCl-injected shrimps. Phenotype, overview | Penaeus monodon |
metabolism | the cholesterol 7,8-dehydrogenase catalyzes the first step of the ecdysteroid biosynthesis converting cholesterol to 7-dehydrocholesterol | Penaeus monodon |
physiological function | function of the enzyme in the molting process and molt regulation, crucial role of PmNvd in molt progression, particularly during the initiation of premolt phase via the regulation of ecdysteroid production | Penaeus monodon |