BRENDA - Enzyme Database
show all sequences of 1.14.19.21

An essential role of Rieske domain oxygenase Neverland in the molting cycle of black tiger shrimp, Penaeus monodon

Sathapondecha, P.; Panyim, S.; Udomkit, A.; Comp. Biochem. Physiol. A 213, 11-19 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis
Penaeus monodon
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction and expression of double-stranded RNA specific to PmNvd (dsNvd) for silencing of the enzyme, effect of PmNvd knockdown on molting duration, phenotype, overview
Penaeus monodon
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme sequence contains the transmembrane motif 33WxxF59
Penaeus monodon
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Penaeus monodon
A0A2I4PEL8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
the expression of PmNvd is dominantly in the Y-organ, but changes during molting period. The PmNvd mRNA level is low in intermolt and early premolt stages, then dramatically increases in the mid premolt stage. No enzyme expression in eyestalk, brain, thoracic ganglia, nerve cord, hepatopancreas, ovary, gills, muscle, and lymphoid organ, RT-PCR expression analysis
Penaeus monodon
-
Y-organ
exclusive expression
Penaeus monodon
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + O2 + NAD(P)H + H+
-
744745
Penaeus monodon
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis
Penaeus monodon
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction and expression of double-stranded RNA specific to PmNvd (dsNvd) for silencing of the enzyme, effect of PmNvd knockdown on molting duration, phenotype, overview
Penaeus monodon
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme sequence contains the transmembrane motif 33WxxF59
Penaeus monodon
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
the expression of PmNvd is dominantly in the Y-organ, but changes during molting period. The PmNvd mRNA level is low in intermolt and early premolt stages, then dramatically increases in the mid premolt stage. No enzyme expression in eyestalk, brain, thoracic ganglia, nerve cord, hepatopancreas, ovary, gills, muscle, and lymphoid organ, RT-PCR expression analysis
Penaeus monodon
-
Y-organ
exclusive expression
Penaeus monodon
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + O2 + NAD(P)H + H+
-
744745
Penaeus monodon
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
silencing of PmNvd transcript in shrimp by specific double-stranded RNA (dsNvd) leads to prolonged molt duration with abnormal molting progression, i.e. the molting process got stuck at early premolt stage. In addition, the 20-hydroxyecdysone titer in hemolymph of dsNvd-injected shrimps is significantly reduced compared with that in NaCl-injected shrimps. Phenotype, overview
Penaeus monodon
metabolism
the cholesterol 7,8-dehydrogenase catalyzes the first step of the ecdysteroid biosynthesis converting cholesterol to 7-dehydrocholesterol
Penaeus monodon
physiological function
function of the enzyme in the molting process and molt regulation, crucial role of PmNvd in molt progression, particularly during the initiation of premolt phase via the regulation of ecdysteroid production
Penaeus monodon
General Information (protein specific)
General Information
Commentary
Organism
malfunction
silencing of PmNvd transcript in shrimp by specific double-stranded RNA (dsNvd) leads to prolonged molt duration with abnormal molting progression, i.e. the molting process got stuck at early premolt stage. In addition, the 20-hydroxyecdysone titer in hemolymph of dsNvd-injected shrimps is significantly reduced compared with that in NaCl-injected shrimps. Phenotype, overview
Penaeus monodon
metabolism
the cholesterol 7,8-dehydrogenase catalyzes the first step of the ecdysteroid biosynthesis converting cholesterol to 7-dehydrocholesterol
Penaeus monodon
physiological function
function of the enzyme in the molting process and molt regulation, crucial role of PmNvd in molt progression, particularly during the initiation of premolt phase via the regulation of ecdysteroid production
Penaeus monodon
Other publictions for EC 1.14.19.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744745
Sathapondecha
An essential role of Rieske d ...
Penaeus monodon
Comp. Biochem. Physiol. A
213
11-19
2017
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1
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5
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1
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1
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1
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2
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1
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3
3
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735284
Poklepovich
Expression and functional char ...
Tetrahymena thermophila
Steroids
96
132-139
2015
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1
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2
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1
6
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7
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6
1
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3
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1
6
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6
1
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725923
Najle
The sterol-C7 desaturase from ...
Tetrahymena thermophila
Mol. Biol. Evol.
30
1630-1643
2013
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8
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10
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11
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11
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723904
Wollam
The Rieske oxygenase DAF-36 fu ...
Caenorhabditis elegans
Aging Cell
10
879-884
2011
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1
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1
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2
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1
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1
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1
1
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725431
Yoshiyama-Yanagawa
The conserved Rieske oxygenase ...
Bombyx mori, Caenorhabditis elegans, Ciona intestinalis, Danio rerio, Hemicentrotus pulcherrimus, Xenopus laevis
J. Biol. Chem.
286
25756-25762
2011
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5
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5
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6
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12
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22
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12
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18
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5
18
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