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Literature summary for 1.14.19.21 extracted from

  • Poklepovich, T.; Urtasun, N.; Miranda, M.; Nusblat, A.; Nudel, C.
    Expression and functional characterization of a C-7 cholesterol desaturase from Tetrahymena thermophila in an insect cell line (2015), Steroids, 96, 132-139.
    View publication on PubMed
Search for more literature for 1.14.19.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Tetrahymena thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane protein Tetrahymena thermophila 16020
-
microsome
-
 Tetrahymena thermophila
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 x * 63000, SDS-PAGE Tetrahymena thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
22-cholesten-3beta-ol + NADH + H+ Tetrahymena thermophila
-
 7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
 ?
22-cholesten-3beta-ol + NADPH + H+ Tetrahymena thermophila
-
 7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
 ?
cholestan-3beta-ol + NADH + H+ Tetrahymena thermophila
-
 7-cholesten-3beta-ol + NAD+ + H2O
-
 ?
cholestan-3beta-ol + NADPH + H+ Tetrahymena thermophila
-
 7-cholesten-3beta-ol + NADP+ + H2O
-
 ?
cholesterol + O2 + NADH + H+ Tetrahymena thermophila
-
 cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
 ?
cholesterol + O2 + NADPH + H+ Tetrahymena thermophila
-
 cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Tetrahymena thermophila I7ML19
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
22-cholesten-3beta-ol + NADH + H+
-
 Tetrahymena thermophila 7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
 ?
22-cholesten-3beta-ol + NADPH + H+
-
 Tetrahymena thermophila 7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
 ?
cholestan-3beta-ol + NADH + H+
-
 Tetrahymena thermophila 7-cholesten-3beta-ol + NAD+ + H2O
-
 ?
cholestan-3beta-ol + NADPH + H+
-
 Tetrahymena thermophila 7-cholesten-3beta-ol + NADP+ + H2O
-
 ?
cholesterol + O2 + NADH + H+
-
 Tetrahymena thermophila cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
 ?
cholesterol + O2 + NADPH + H+
-
 Tetrahymena thermophila cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Tetrahymena thermophila

Synonyms

Synonyms Comment Organism
C-7 cholesterol desaturase
-
 Tetrahymena thermophila
Des7
-
 Tetrahymena thermophila
Des7p
-
 Tetrahymena thermophila

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Tetrahymena thermophila
NADPH
-
 Tetrahymena thermophila
[2Fe-2S]-center
-
 Tetrahymena thermophila