BRENDA - Enzyme Database show
show all sequences of 1.14.19.21

Expression and functional characterization of a C-7 cholesterol desaturase from Tetrahymena thermophila in an insect cell line

Poklepovich, T.; Urtasun, N.; Miranda, M.; Nusblat, A.; Nudel, C.; Steroids 96, 132-139 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Sf9 insect cells
Tetrahymena thermophila
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane protein
Tetrahymena thermophila
16020
-
microsome
-
Tetrahymena thermophila
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
x * 63000, SDS-PAGE
Tetrahymena thermophila
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
22-cholesten-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
?
22-cholesten-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
?
cholestan-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NAD+ + H2O
-
-
?
cholestan-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Tetrahymena thermophila
I7ML19
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
22-cholesten-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
-
?
22-cholesten-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
-
?
cholestan-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NAD+ + H2O
-
-
-
?
cholestan-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 63000, SDS-PAGE
Tetrahymena thermophila
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Tetrahymena thermophila
NADPH
-
Tetrahymena thermophila
[2Fe-2S]-center
-
Tetrahymena thermophila
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Sf9 insect cells
Tetrahymena thermophila
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Tetrahymena thermophila
NADPH
-
Tetrahymena thermophila
[2Fe-2S]-center
-
Tetrahymena thermophila
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane protein
Tetrahymena thermophila
16020
-
microsome
-
Tetrahymena thermophila
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
x * 63000, SDS-PAGE
Tetrahymena thermophila
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
22-cholesten-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
?
22-cholesten-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
?
cholestan-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NAD+ + H2O
-
-
?
cholestan-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
22-cholesten-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
-
?
22-cholesten-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
-
?
cholestan-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NAD+ + H2O
-
-
-
?
cholestan-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 63000, SDS-PAGE
Tetrahymena thermophila
Other publictions for EC 1.14.19.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744745
Sathapondecha
An essential role of Rieske d ...
Penaeus monodon
Comp. Biochem. Physiol. A
213
11-19
2017
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-
1
-
1
-
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-
1
1
-
-
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5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
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-
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-
1
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
735284
Poklepovich
Expression and functional char ...
Tetrahymena thermophila
Steroids
96
132-139
2015
-
-
1
-
-
-
-
-
2
-
1
6
-
6
-
-
-
-
-
-
-
-
6
1
-
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-
-
-
-
-
3
-
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-
1
3
-
-
-
-
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2
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1
6
-
-
-
-
-
-
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6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725923
Najle
The sterol-C7 desaturase from ...
Tetrahymena thermophila
Mol. Biol. Evol.
30
1630-1643
2013
-
-
-
-
-
-
-
-
-
-
-
8
-
10
-
-
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-
-
-
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11
-
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-
-
-
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3
-
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3
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723904
Wollam
The Rieske oxygenase DAF-36 fu ...
Caenorhabditis elegans
Aging Cell
10
879-884
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725431
Yoshiyama-Yanagawa
The conserved Rieske oxygenase ...
Bombyx mori, Caenorhabditis elegans, Ciona intestinalis, Danio rerio, Hemicentrotus pulcherrimus, Xenopus laevis
J. Biol. Chem.
286
25756-25762
2011
-
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5
-
5
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6
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12
-
22
-
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-
-
-
-
-
12
-
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-
-
-
-
-
-
18
-
-
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-
5
18
-
5
-
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-
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6
-
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12
-
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12
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