BRENDA - Enzyme Database
show all sequences of 1.14.19.21

The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme

Yoshiyama-Yanagawa, T.; Enya, S.; Shimada-Niwa, Y.; Yaguchi, S.; Haramoto, Y.; Matsuya, T.; Shiomi, K.; Sasakura, Y.; Takahashi, S.; Asashima, M.; Kataoka, H.; Niwa, R.; J. Biol. Chem. 286, 25756-25762 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Drosophila melanogaster S2 cells
Bombyx mori
expressed in Drosophila melanogaster S2 cells
Caenorhabditis elegans
expressed in Drosophila melanogaster S2 cells
Danio rerio
expressed in Drosophila melanogaster S2 cells
Hemicentrotus pulcherrimus
expressed in Drosophila melanogaster S2 cells
Xenopus laevis
Engineering
Amino acid exchange
Commentary
Organism
C122A
inactive
Caenorhabditis elegans
C178A
inactive
Xenopus laevis
D234A
inactive
Caenorhabditis elegans
H190A
inactive
Bombyx mori
H282A
inactive
Bombyx mori
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bombyx mori
-
-
microsome
-
Caenorhabditis elegans
-
-
microsome
-
Ciona intestinalis
-
-
microsome
-
Danio rerio
-
-
microsome
-
Hemicentrotus pulcherrimus
-
-
microsome
-
Xenopus laevis
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + O2 + NADH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bombyx mori
Q1JUZ2
-
-
Caenorhabditis elegans
Q17938
-
-
Ciona intestinalis
F7J186
-
-
Danio rerio
Q6DHJ3
-
-
Hemicentrotus pulcherrimus
F7J188
-
-
Xenopus laevis
F7J184
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + O2 + NADH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Bombyx mori
NADH
-
Caenorhabditis elegans
NADH
-
Ciona intestinalis
NADH
-
Danio rerio
NADH
-
Hemicentrotus pulcherrimus
NADH
-
Xenopus laevis
NADPH
-
Bombyx mori
NADPH
-
Caenorhabditis elegans
NADPH
-
Ciona intestinalis
NADPH
-
Danio rerio
NADPH
-
Hemicentrotus pulcherrimus
NADPH
-
Xenopus laevis
[2Fe-2S]-center
-
Bombyx mori
[2Fe-2S]-center
-
Caenorhabditis elegans
[2Fe-2S]-center
-
Ciona intestinalis
[2Fe-2S]-center
-
Danio rerio
[2Fe-2S]-center
-
Hemicentrotus pulcherrimus
[2Fe-2S]-center
-
Xenopus laevis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Drosophila melanogaster S2 cells
Bombyx mori
expressed in Drosophila melanogaster S2 cells
Caenorhabditis elegans
expressed in Drosophila melanogaster S2 cells
Danio rerio
expressed in Drosophila melanogaster S2 cells
Hemicentrotus pulcherrimus
expressed in Drosophila melanogaster S2 cells
Xenopus laevis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Bombyx mori
NADH
-
Caenorhabditis elegans
NADH
-
Ciona intestinalis
NADH
-
Danio rerio
NADH
-
Hemicentrotus pulcherrimus
NADH
-
Xenopus laevis
NADPH
-
Bombyx mori
NADPH
-
Caenorhabditis elegans
NADPH
-
Ciona intestinalis
NADPH
-
Danio rerio
NADPH
-
Hemicentrotus pulcherrimus
NADPH
-
Xenopus laevis
[2Fe-2S]-center
-
Bombyx mori
[2Fe-2S]-center
-
Caenorhabditis elegans
[2Fe-2S]-center
-
Ciona intestinalis
[2Fe-2S]-center
-
Danio rerio
[2Fe-2S]-center
-
Hemicentrotus pulcherrimus
[2Fe-2S]-center
-
Xenopus laevis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C122A
inactive
Caenorhabditis elegans
C178A
inactive
Xenopus laevis
D234A
inactive
Caenorhabditis elegans
H190A
inactive
Bombyx mori
H282A
inactive
Bombyx mori
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bombyx mori
-
-
microsome
-
Caenorhabditis elegans
-
-
microsome
-
Ciona intestinalis
-
-
microsome
-
Danio rerio
-
-
microsome
-
Hemicentrotus pulcherrimus
-
-
microsome
-
Xenopus laevis
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + O2 + NADH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
cholesterol + O2 + NADPH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + O2 + NADH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
cholesterol + O2 + NADPH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.19.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744745
Sathapondecha
An essential role of Rieske d ...
Penaeus monodon
Comp. Biochem. Physiol. A
213
11-19
2017
-
-
1
-
1
-
-
-
1
1
-
-
-
5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
735284
Poklepovich
Expression and functional char ...
Tetrahymena thermophila
Steroids
96
132-139
2015
-
-
1
-
-
-
-
-
2
-
1
6
-
7
-
-
-
-
-
-
-
-
6
1
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-
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3
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1
3
-
-
-
-
-
-
-
2
-
1
6
-
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6
1
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-
-
-
-
-
-
-
-
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-
-
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-
725923
Najle
The sterol-C7 desaturase from ...
Tetrahymena thermophila
Mol. Biol. Evol.
30
1630-1643
2013
-
-
-
-
-
-
-
-
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-
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8
-
10
-
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11
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3
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3
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8
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11
-
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-
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-
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-
-
-
-
-
723904
Wollam
The Rieske oxygenase DAF-36 fu ...
Caenorhabditis elegans
Aging Cell
10
879-884
2011
-
-
1
-
-
-
-
-
-
-
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1
-
2
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1
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1
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1
1
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1
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1
-
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-
-
-
-
-
-
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1
1
-
-
-
725431
Yoshiyama-Yanagawa
The conserved Rieske oxygenase ...
Bombyx mori, Caenorhabditis elegans, Ciona intestinalis, Danio rerio, Hemicentrotus pulcherrimus, Xenopus laevis
J. Biol. Chem.
286
25756-25762
2011
-
-
5
-
5
-
-
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6
-
-
12
-
22
-
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-
-
-
-
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12
-
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-
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18
-
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5
18
-
5
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6
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12
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12
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