Literature summary for 1.14.19.2 extracted from

Du, H.; Huang, M.; Hu, J.; Li, J.
Modification of the fatty acid composition in Arabidopsis and maize seeds using a stearoyl-acyl carrier protein desaturase-1 (ZmSAD1) gene (2016), BMC Plant Biol., 16, 137 .

Search for more literature for 1.14.19.2

Cloned(Commentary)

Cloned (Comment)	Organism
gene SAD1, phylogenetic analysis	Arabidopsis thaliana
gene SAD1, phylogenetic analysis, ZmSAD1 overexpression (ZmSAD1), antisense ZmSAD1 (anti-ZmSAD1), and ZmSAD1 RNA interference (ZmSAD1 RNAi) constructs are generated in the pBI121 vector under control of the seed-specific FAE1 promoter, recombinant expression in Arabidopsis thaliana with higher expression levels in mature seeds and immature siliques than in roots, stems, leaves, and petals. Fatty acid composition and content are modified in the transgenic Arabidopsis seeds, seed-specific overexpression of the exogenous ZmSAD1 gene significantly reduces the content of stearic acid and the ratio of saturated to unsaturated fatty acids, composition of fatty acids in the Arabidopsis seeds harbouring the exogenous ZmSAD1, anti-ZmSAD1, or ZmSAD1 RNAi constructs. Phenotypes, overview	Zea mays

Cloned (Comment)

Organism

gene SAD1, phylogenetic analysis

Arabidopsis thaliana

gene SAD1, phylogenetic analysis, ZmSAD1 overexpression (ZmSAD1), antisense ZmSAD1 (anti-ZmSAD1), and ZmSAD1 RNA interference (ZmSAD1 RNAi) constructs are generated in the pBI121 vector under control of the seed-specific FAE1 promoter, recombinant expression in Arabidopsis thaliana with higher expression levels in mature seeds and immature siliques than in roots, stems, leaves, and petals. Fatty acid composition and content are modified in the transgenic Arabidopsis seeds, seed-specific overexpression of the exogenous ZmSAD1 gene significantly reduces the content of stearic acid and the ratio of saturated to unsaturated fatty acids, composition of fatty acids in the Arabidopsis seeds harbouring the exogenous ZmSAD1, anti-ZmSAD1, or ZmSAD1 RNAi constructs. Phenotypes, overview

Zea mays

Protein Variants

Protein Variants	Comment	Organism
additional information	ZmSAD1 overexpression (ZmSAD1), antisense ZmSAD1 (anti-ZmSAD1), and ZmSAD1 RNA interference (ZmSAD1 RNAi) constructs are generated in the pBI121 vector under control of the seed-specific FAE1 promoter. Ttransformation of the ZmSAD1 and ZmSAD1 RNAi constructs under control ofthe FAE1 promoter into maize by particle bombardment. Overexpression of ZmSAD1 changes the fatty acid composition in maize seeds, expression of seed-specific ZmSAD1 results in a decrease in the content of stearic and other saturated acids. Composition of fatty acids in the maize seeds harbouring ZmSAD1 or ZmSAD1 RNAi constructs, phenotypes, overview	Zea mays

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LF04	-	-
Zea mays	B4F9Q1	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Arabidopsis thaliana	-
kernel	-	Zea mays	-
seed	-	Arabidopsis thaliana	-
seed	expression levels of ZmSAD1 in the mature maize embryos, ten maize embryos are excised from the mature seeds, overview	Zea mays	-
seedling	-	Zea mays	-

Synonyms

Synonyms	Comment	Organism
AtSAD1	-	Arabidopsis thaliana
SAD1	-	Arabidopsis thaliana
SAD1	-	Zea mays
stearoyl-acyl carrier protein desaturase-1	-	Arabidopsis thaliana
stearoyl-acyl carrier protein desaturase-1	-	Zea mays
ZmSAD1	-	Zea mays

Expression

Organism	Comment	Expression
Arabidopsis thaliana	seed-specific down-regulation of endogenous AtSAD genes in Arabidopsis significantly increases the content of stearic and long-chain saturated fatty acids and the ratio of saturated to unsaturated fatty acids, whereas it significantly decreases the oleic acid content. Composition of fatty acids in the Arabidopsis seeds harbouring the exogenous ZmSAD1, anti-ZmSAD1, or ZmSAD1 RNAi constructs, overview	additional information

General Information

General Information	Comment	Organism
malfunction	transgenic maize that expresses high levels of ZmSAD1 in its mature seeds shows reduced stearic acid content (1.57%) and a lower saturated to unsaturated fatty acid ratio (20.40%) relative to those (1.64% and 20.61%, respectively) of the control. Conversely, downregulation of ZmSAD1 in maize results in increased levels of stearic acid (1.78%), long-chain saturated acids (0.85%) and the ratio of saturated to unsaturated fatty acids (21.54%) relative to those (1.64%, 0.74%, and 20.61%, respectively) of the control, whereas the oleic acid (32.01%) level is significantly decreased relative to that (32.68%) of the control	Zea mays
additional information	seed-specific overexpression of the exogenous ZmSAD1 gene in Arabidopsis thaliana significantly reduces the content of stearic acid and the ratio of saturated to unsaturated fatty acids	Arabidopsis thaliana