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Literature summary for 1.14.19.2 extracted from
- Engineering D9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor DELTA9-18:0-ACP desaturase (2001), J. Biol. Chem., 276, 21500-21505.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T117R/G188L | 82fold higher specificity for palmitoyl-[acyl-carrier protein] with respect to wild-type | Ricinus communis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00046 | - |
octadecanoyl-[acyl-carrier protein] | wild type enzyme, pH and temperature not specified in the publication | Ricinus communis |  |
| 0.00055 | - |
hexadecanoyl-[acyl-carrier protein] | T117R/G188L mutant enzyme | Ricinus communis | |
| 0.00055 | - |
hexadecanoyl-[acyl-carrier protein] | mutant enzyme T117R/G188L, pH and temperature not specified in the publication | Ricinus communis | |
| 0.00098 | - |
octadecanoyl-[acyl-carrier protein] | T117R/G188L mutant enzyme | Ricinus communis | |
| 0.00098 | - |
octadecanoyl-[acyl-carrier protein] | mutant enzyme T117R/G188L, pH and temperature not specified in the publication | Ricinus communis | |
| 0.001 | - |
tetradecanoyl-[acyl-carrier protein] | T117R/G188L mutant enzyme | Ricinus communis | |
| 0.00464 | - |
tetradecanoyl-[acyl-carrier protein] | - |
Ricinus communis | |
| 0.005 | - |
hexadecanoyl-[acyl-carrier protein] | wild type enzyme, pH and temperature not specified in the publication | Ricinus communis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | P22337 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2 + H+ | - |
Ricinus communis | 9-hexadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O | - |
r | |
| octadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2 + H+ | - |
Ricinus communis | 9-octadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O | - |
r | |
| tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Ricinus communis | 9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
tetradecanoyl-[acyl-carrier protein] | - |
Ricinus communis | |
| 0.0273 | - |
tetradecanoyl-[acyl-carrier protein] | T117R/G188L mutant enzyme | Ricinus communis | |
| 0.0467 | - |
hexadecanoyl-[acyl-carrier protein] | - |
Ricinus communis | |
| 0.0817 | - |
octadecanoyl-[acyl-carrier protein] | T117R/G188L mutant enzyme | Ricinus communis | |
| 0.422 | - |
hexadecanoyl-[acyl-carrier protein] | T117R/G188L mutant enzyme | Ricinus communis | |
| 0.705 | - |
octadecanoyl-[acyl-carrier protein] | - |
Ricinus communis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Ricinus communis |
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