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Literature summary for 1.14.19.2 extracted from
- Fat metabolism in higher plants. Properties of a soluble stearyl-acyl carrier protein desaturase from maturing Carthamus tinctorius (1974), Arch. Biochem. Biophys., 162, 158-165.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | enhances activity | Carthamus tinctorius |  |
| reduced glutathione | enhances activity | Carthamus tinctorius | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-mercaptoethanol | - |
Carthamus tinctorius | |
| cytochrome c3 | - |
Carthamus tinctorius | |
| cytochrome c553 | - |
Carthamus tinctorius | |
| FAD | - |
Carthamus tinctorius | |
| FMN | - |
Carthamus tinctorius | |
| KCN | 1 mM, strong inhibition | Carthamus tinctorius | |
| additional information | not inhibited by CO | Carthamus tinctorius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
NADPH | - |
Carthamus tinctorius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Carthamus tinctorius | 5737 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carthamus tinctorius | P22243 | safflower | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | specific for stearoyl-CoA | Carthamus tinctorius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Carthamus tinctorius | - |
| seed | - |
Carthamus tinctorius | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| stearoyl-CoA + electron donor + O2 | - |
Carthamus tinctorius | 9-octadecenoyl-CoA + acceptor + H2O | - |
? | |
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | no activity with NADH | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | electron donor NADPH | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | a system composed of ferredoxin, grana lamellae, ascorbic acid, dichlorophenolindophenol and light is the most effective reductant | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | enzyme is specific for stearoyl-CoA | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | required for activity | Carthamus tinctorius | |
| additional information | NADH is ineffective as electron donor | Carthamus tinctorius | |
| NADPH | - |
Carthamus tinctorius | |
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Release 2023.1 (January 2023)
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