BRENDA - Enzyme Database show
show all sequences of 1.14.19.12

A front-end desaturase from Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by omega13 desaturation in methylotrophic yeast and tobacco

Kajikawa, M.; Yamato, K.; Kohzu, Y.; Shoji, S.; Matsui, K.; Tanaka, Y.; Sakai, Y.; Fukuzawa, H.; Plant Cell Physiol. 47, 64-73 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
DELTA5-desaturase activity
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
DELTA5-desaturase activity
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
low activity
?
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
low activity
?
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
low activity
?
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
low activity
?
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
preferred substrate, DELTA5-desaturase activity
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
preferred substrate, DELTA5-desaturase activity
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
additional information
Chlamydomonas reinhardtii
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
?
-
-
-
additional information
Chlamydomonas reinhardtii 137C
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
?
-
-
-
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
low activity
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlamydomonas reinhardtii
Q2HWK7
-
-
Chlamydomonas reinhardtii 137C
Q2HWK7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii 137C
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii
?
-
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii 137C
?
-
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii
?
-
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii 137C
?
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
preferred substrate, DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
preferred substrate, DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii 137C
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
additional information
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
734881
Chlamydomonas reinhardtii
?
-
-
-
-
additional information
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
734881
Chlamydomonas reinhardtii 137C
?
-
-
-
-
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii
?
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Chlamydomonas reinhardtii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris
Chlamydomonas reinhardtii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
DELTA5-desaturase activity
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
DELTA5-desaturase activity
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
low activity
?
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
low activity
?
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
low activity
?
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
low activity
?
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
preferred substrate, DELTA5-desaturase activity
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii 137C
preferred substrate, DELTA5-desaturase activity
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
additional information
Chlamydomonas reinhardtii
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
?
-
-
-
additional information
Chlamydomonas reinhardtii 137C
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
?
-
-
-
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Chlamydomonas reinhardtii
low activity
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii 137C
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii
?
-
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii 137C
?
-
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii
?
-
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii 137C
?
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
preferred substrate, DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
preferred substrate, DELTA5-desaturase activity
734881
Chlamydomonas reinhardtii 137C
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
additional information
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
734881
Chlamydomonas reinhardtii
?
-
-
-
-
additional information
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
734881
Chlamydomonas reinhardtii 137C
?
-
-
-
-
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
low activity
734881
Chlamydomonas reinhardtii
?
-
-
-
?
Other publictions for EC 1.14.19.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734881
Kajikawa
A front-end desaturase from Ch ...
Chlamydomonas reinhardtii 137C, Chlamydomonas reinhardtii
Plant Cell Physiol.
47
64-73
2006
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