Cloned (Comment) | Organism |
---|---|
gene scd1, quantitative real-time PCR enzyme expression analysis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-chlorophenoxy)-N-[3-[(methylamino)carbonyl]phenyl]-1-piperidinecarboxamide | i.e. A939 or A939572, an SCD1 inhibitor, causes significantly delayed tumor growth, which is reversed by L-cycloserine, an inhibitor of ceramide biosynthesis. A939 can significantly inhibit LOVO cell proliferation, which can be dose-dependently reversed by oleic acid, but not by palmitic acid | Homo sapiens | |
Cay10566 | i.e. Cay105 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | mainly | Homo sapiens | 5783 | - |
membrane | transmembrane enzyme | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Homo sapiens | - |
palmitoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Homo sapiens | - |
oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00767 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Colo-205 cell | - |
Homo sapiens | - |
colon | - |
Homo sapiens | - |
colorectal cancer cell | - |
Homo sapiens | - |
LoVo cell | colorectal adenocarcinoma cells | Homo sapiens | - |
additional information | mRNA expression levels of SCD1 in tumor tissues markedly elevated compared to that in adjacent non-tumor tissues | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | palmitoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCD1 | - |
Homo sapiens |
stearoyl-CoA desaturase 1 | - |
Homo sapiens |
stearoyl-CoA desaturase-1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | reduction of SCD1 activity and mRNA expression impaired the formation of cell membrane lipids with the decrease of fatty acid biosynthesis and desaturation, leading to cease cancer cell proliferation and induction of cell apoptosis. Inhibition of stearoyl-CoA desaturase 1 (SCD1) effectively suppresses tumor cell proliferation and induce apoptosis in numerous neoplastic lesions, SCD1-mediated anti-tumor effect. Endo-lipid messenger ceramides play a critical role in tumor fate modulated by SCD1 inhibition. Inhibition of SCD1 activity promotes apoptosis attributed to mitochondria dysfunctions, upregulation of reaction oxygen species (ROS), alteration of mitochondrial transmembrane potential, and translocation of mitochondrial protein cytochrome C in colorectal cancer cells. These effects are mediated by intracellular ceramide signals through induction of ceramide biosynthesis, rather than exclusive saturated fatty acid accumulation. Inhibitor A939 dramatically increases intracellular long-chain saturated ceramide levels including C18:0 ceramide, C20:0 ceramide, C22:0 ceramide, and C24:0 ceramide, while it slightly increases middle-chain saturated ceramide C16:0 and does not affect monounsaturated C24:1 ceramide | Homo sapiens |
metabolism | cross-talk of SCD1-mediated lipid pathway and endo-ceramide biosynthesis pathway, indicating roles of ceramide signals in SCD1-mediated anti-tumor property | Homo sapiens |
physiological function | stearoyl-CoA desaturase-1 (SCD1) catalyzes saturated fatty acids to DELTA-9 monounsaturated fatty acids, e.g. converting palmitic acid (C16:0) to palmitoleic acid (C16:1) or converting stearic acid (C18:0) to oleic acid (C18:1). SCD1 is necessary to stimulate lipid biosynthesis to supply new phospholipids for cell membrane biogenesis in cell cycle process of mitosis | Homo sapiens |