Literature summary for 1.14.19.1 extracted from

Goren, M.A.; Fox, B.G.
Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex (2008), Protein Expr. Purif., 62, 171-178.

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Cloned(Commentary)

Cloned (Comment)	Organism
in vitro translation using a wheat germ cell-free extract in the presence of unilamelar liposomes, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, overview	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	heme and non-heme iron	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
stearoyl-CoA + ferrocytochrome b5 + O2 + H+	Homo sapiens	-	oleoyl-CoA + ferricytochrome b5 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O00767	-	-

Purification (Commentary)

Purification (Comment)	Organism
near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes, overview	Homo sapiens

Purification (Comment)

Organism

near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes, overview

Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
in vitro reconstitution of the non-heme iron and heme active sites to a functional reconstituted enzyme complex after in vitro translation and protein integration into liposomes, method optimization, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
stearoyl-CoA + ferrocytochrome b5 + O2 + H+	-	Homo sapiens	oleoyl-CoA + ferricytochrome b5 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
SCD	-	Homo sapiens
stearoyl-CoA desaturase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ferrocytochrome b5	-	Homo sapiens