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Literature summary for 1.14.19.1 extracted from
- A desaturase-like protein from white spruce is a DELTA9 desaturase (2002), FEBS Lett., 526, 49-52.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologous gene expression in yeast | Picea glauca |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | yeast expression strain carries a mutation in the stearoyl-CoA desaturase gene | Picea glauca |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Picea glauca | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43700 | - |
x * 43700, calculated | Picea glauca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picea glauca | - |
white pruce | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | regioselectivity, substrate specificity, probably extra-plastidal | Picea glauca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Picea glauca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0002375 | - |
- |
Picea glauca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | glycerolipid such as phosphatidylcholine is involved as an intermediate substrate | Picea glauca | ? | - |
? |  |
| palmitic acid + AH2 + O2 | - |
Picea glauca | palmitoleic acid + A + H2O | - |
? | |
| stearate + AH2 + O2 | - |
Picea glauca | oleate + A + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43700, calculated | Picea glauca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Picea glauca |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | no evidence for | Picea glauca | |
| NADH | - |
Picea glauca | |
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Release 2023.1 (January 2023)
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