Literature summary for 1.14.18.9 extracted from

Oger, E.; Ghignone, S.; Campagnac, E.; Fontaine, J.; Grandmougin-Ferjani, A.; Lanfranco, L.
Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices (2009), Fungal Genet. Biol., 46, 486-495.

Search for more literature for 1.14.18.9

Cloned(Commentary)

Cloned (Comment)	Organism
DNA is extracted from extraradial mycelium growing around Cichorium intybus, PCR-amplification, cloned into pGEM-T Vector System for sequencing, for complementation assay in Saccharomyces cerevisiae mutant erg25-25c (methyl oxidase deficient) the GintSMO gene is amplified and cloned into the NotI-linearized yeast expression vector pFL61	Rhizophagus intraradices

Protein Variants

Protein Variants	Comment	Organism
additional information	in a complementation assay, the introduced GintSMO gene in methyl oxidase deficient Saccharomyces cerevisiae mutant erg25-25c restores growth in the absence of ergosterol (endproduct of the sterol biosynthesic pathway in the yeast)	Rhizophagus intraradices

Inhibitors

Inhibitors	Comment	Organism	Structure
fenhexamid	fungicide, sterol biosynthesis inhibitor, 9.3 mg/l growth medium (long term exposure for 8 weeks) inhibits germ tube length by 50%, no change in enzyme transcription (0.2-2 mg/l growth medium)	Rhizophagus intraradices
fenpropimorph	fungicide, sterol biosynthesis inhibitor, 6.4 mg/l growth medium (long term exposure for 8 weeks) inhibits germ tube length by 50%, strong decrease in transcription levels with 2 mg/l growth medium, 10 mg/l decreases the fraction of vital stained fungal cells (after 2 and 7 days of treatment), leads to a increase in enzyme mRNA, does not change relative sterol composition and sterol amounts but reduced squalen content by 87% after 7 days of treatment	Rhizophagus intraradices

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Rhizophagus intraradices	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	calculated from amino acids	Rhizophagus intraradices

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rhizophagus intraradices	squalene is sterol precursor, 24-methyl and 24-ethyl cholesterol are major sterol compounds in arbuscular mycorrhizal fungi, biosynthetic intermediates are lanosterol and 24-methylene lanosterol	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhizophagus intraradices	C5HVZ4	endomycorrhizal fungus, grown on chicory (Cichorium intybus) roots	-

Purification (Commentary)

Purification (Comment)	Organism
extraradial mycelium collected from solubilized medium in buffer (50 mM Tris-HCl, pH 7.5, 10 mM EDTA), vacuum filtrated, frozen in liquid nitrogen	Rhizophagus intraradices

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	squalene is sterol precursor, 24-methyl and 24-ethyl cholesterol are major sterol compounds in arbuscular mycorrhizal fungi, biosynthetic intermediates are lanosterol and 24-methylene lanosterol	Rhizophagus intraradices	?	-	?

Synonyms

Synonyms	Comment	Organism
C-4 sterol methyl oxidase	-	Rhizophagus intraradices
GintSMO protein	-	Rhizophagus intraradices
SMO	-	Rhizophagus intraradices

General Information

General Information	Comment	Organism
metabolism	first of three steps to remove 2 C4 methyl groups from an intermediate in ergosterol biosynthesis in the sterol biosynthetic pathway, an enzymatic complex is formed with other enzymes in the pathway	Rhizophagus intraradices

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Release 2023.1 (January 2023)