Literature summary for 1.14.18.4 extracted from

Venegas-Caleron, M.; Sanchez, R.; Salas, J.J.; Garces, R.; Martinez-Force, E.
Molecular and biochemical characterization of the OLE-1 high-oleic castor seed (Ricinus communis L.) mutant (2016), Planta, 244, 245-258 .

Search for more literature for 1.14.18.4

Cloned(Commentary)

Cloned (Comment)	Organism
gene RcFAH12, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain W303-1A, fatty acid analysis	Ricinus communis

Protein Variants

Protein Variants	Comment	Organism
F49S/V242A/H319Q	naturally occuring mutation in the OLE-1 mutant strain i.e. high-oleic castor mutant. Residue F49 is located in domain I, residue V242 in transmembrane domain TM3, and residue H319 in the His box III or domain IX, respectively, In the high-oleic mutant seed, embryos mostly accumulate palmitic, oleic and linoleic in the first stages of development, which is followed by an important accumulation of oleic acid during seed maturation. The ricinoleic acid content remains low in these seeds throughout development, in contrast to the wild-type seeds. Comparison of fatty acid profiles of seeds. The mutant displays a phenotype similar to the control cells that are transformed with the empty pYES2 plasmid. Structural effect of the mutations, overview	Ricinus communis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	an integral membrane protein, proposed topological model, overview	Ricinus communis	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cis-oleic acid + reduced acceptor + O2	Ricinus communis	-	ricinoleic acid + acceptor + H2O	-	?
cis-oleic acid + reduced acceptor + O2	Ricinus communis OLE-1	-	ricinoleic acid + acceptor + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Ricinus communis	Q41131	wild-type and OLE-1 mutant strain i.e. high-oleic castor mutant	-
Ricinus communis OLE-1	Q41131	wild-type and OLE-1 mutant strain i.e. high-oleic castor mutant	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	fatty acid profiles during the development of wild-type castor bean seeds and OLE-1 mutant seeds, overview	Ricinus communis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cis-oleic acid + reduced acceptor + O2	-	Ricinus communis	ricinoleic acid + acceptor + H2O	-	?
cis-oleic acid + reduced acceptor + O2	-	Ricinus communis OLE-1	ricinoleic acid + acceptor + H2O	-	?

Subunits

Subunits	Comment	Organism
More	topology predictions for the castor DELTA12-hydroxylase. The RcFAH12 sequence is divided into nine consecutive domains from I through to IX: domain I is the cytosolic N-terminal region, domain II is two transmembrane helices (TM1 and TM2) connected by a short ER luminal loop, domain III is a short cytosolic loop containing the first histidine box that is involved in the catalytic site, domain IV is the first peripheral membrane-associated segment (PMS1), domain V is the second short cytosolic loop containing the second histidine box, domain VI is the second peripheral membrane-associated region (PMS2), domain VII is the third short cytosolic loop, domain VIII is the second group of transmembrane helices (TM3 and TM4) that are connected by a short ER luminal loop, and domain IX is the cytosolic C terminus containing the third histidine motif	Ricinus communis

Synonyms

Synonyms	Comment	Organism
castor DELTA12-hydroxylase	-	Ricinus communis
FAH12	-	Ricinus communis
oleate 12-hydroxylase	-	Ricinus communis

General Information

General Information	Comment	Organism
physiological function	common castor bean seed lipids contain high levels of palmitic and linoleic acid in the initial stages of development. As development proceeds, the ricinoleic acid content constitutes up to 80% of the total fatty acids in mature seeds of the wild-type. In the high-oleic mutant seed, embryos mostly accumulate palmitic, oleic and linoleic in the first stages of development, which is followed by an important accumulation of oleic acid during seed maturation. The ricinoleic acid content remains low in these seeds throughout development	Ricinus communis

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Release 2023.1 (January 2023)