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Literature summary for 1.14.18.4 extracted from
- Suppression of ricinoleic acid toxicity by ptl2 overexpression in fission yeast Schizosaccharomyces pombe (2014), Appl. Microbiol. Biotechnol., 98, 9325-9337 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FAH12, recombinant overexpression in Schizosaccharomyces pombe strain ARC010-1, RT-PCR enzyme expression analysis. The enzyme product is toxic for the cells. Gene plg7, encoding phospholipase A2, acts as a multicopy suppressor restoring the growth defect by removing ricinoleic acid from phospholipids and inducing secretion of a part of the released free ricinoleic acid into culture media | Claviceps purpurea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | optimization of the recombinant enzyme from gene FAH12 overexpression in Schizosaccharomyces pombe, overview. The enzyme product is toxic for the yeast cells. Gene plg7, encoding phospholipase A2, acts as a multicopy suppressor restoring the growth defect by removing ricinoleic acid from phospholipids and inducing secretion of a part of the released free ricinoleic acid into culture media. Overexpression of Schizosaccharomyces pombe triglyceride (TG) lipase encoded by gene ptl2, but not the expression of the TG lipases encodd by ptl1 and ptl3, suppresses the growth defect induced by ricinoleic acid production, and the culture grown at 20°C secretes free ricinoleic acid into media like plg7 overexpression. Suppression by ptl2 is independent of plg7, and a large amount of free ricinoleic acid is accumulated in the cells concomitant with the decrease in ricinoleic acid moieties in phospholipids. The suppression by ptl2 is attenuated by bromoenol lactone (BEL), a phospholipase A2 specific inhibitor, suggesting that Ptl2p may have phospholipase activity. Simultaneous overexpression of ptl2 and plg7 in the FAH12 integrant increases secretion and intracellular accumulation of ricinoleic acid 1.2 and 1.3fold, respectively, compared to those with single overexpression of plg7 on day 10 at 20°C. Method optimization and evaluation, overview | Claviceps purpurea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-oleic acid + reduced acceptor + O2 | Claviceps purpurea | - |
ricinoleic acid + acceptor + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Claviceps purpurea | B4YQU1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-oleic acid + reduced acceptor + O2 | - |
Claviceps purpurea | ricinoleic acid + acceptor + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CpFAH12 | - |
Claviceps purpurea |
| oleate DELTA12-hydroxylase | - |
Claviceps purpurea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Claviceps purpurea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Claviceps purpurea |
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Release 2023.1 (January 2023)
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