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Literature summary for 1.14.18.3 extracted from

  • Rosenzweig, A.C.
    The metal centres of particulate methane mono-oxygenase (2008), Biochem. Soc. Trans., 36, 1134-1137.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.18.3

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.8 A resolution Methylococcus capsulatus
to 3.9 A resolution Methylosinus trichosporium OB3b

Inhibitors

Inhibitors Comment Organism Structure
copper copper contents per 100 kDa alphabetagamma protomer of 15-20, eight to ten, two to three and two. Mixture of Cu(I) and Cu(II), copper cluster with a short Cu-Cu distance of 2.51 A that increases to 2.65 A upon chemical reduction with dithionite. The pMMO contains a mononuclear type 2Cu(II) centre and some type of copper cluster Methylococcus capsulatus
copper contains approximately two copper ions per 100 kDa protomer, type 2 Cu(II) present as two distinct species, mixture of Cu(I) and Cu(II). Short Cu-Cu interaction at 2.51 A. Di-copper centre plays an important functional role in pMMO, whereas the mononuclear copper centre is not critical Methylosinus trichosporium OB3b
Fe2+ 0.75 to two iron ions per protomer. Di-iron may be the pMMO active site Methylococcus capsulatus
additional information no detectable iron Methylosinus trichosporium OB3b
Zinc site within the membrane that can be occupied by zinc or copper Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methylosinus trichosporium OB3b 16020
-
membrane
-
 Methylococcus capsulatus 16020
-

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus G1UBD1
-
-
Methylococcus capsulatus Bath G1UBD1
-
-
Methylosinus trichosporium OB3b
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus Bath methanol + acceptor + H2O
-
 ?

Synonyms

Synonyms Comment Organism
particulate methane mono-oxygenase
-
 Methylosinus trichosporium OB3b
particulate methane mono-oxygenase
-
 Methylococcus capsulatus
pMMO
-
 Methylosinus trichosporium OB3b
pMMO
-
 Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Methylococcus capsulatus
NADPH
-
 Methylococcus capsulatus