Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | sMMO | Methylococcus capsulatus | 5737 | - |
membrane | bound, pMMO | Methylococcus capsulatus | 16020 | - |
additional information | two distinct enzyme forms exist: a soluble cytoplasmic sMMO and a membrane-bound particulate pMMO | Methylococcus capsulatus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | required, activates | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
26000 | - |
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
47000 | - |
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
390000 | - |
gel filtration | Methylococcus capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | Methylococcus capsulatus | - |
methanol + acceptor + H2O | - |
? | |
methane + reduced acceptor + H* + O2 | Methylococcus capsulatus Bath | - |
methanol + acceptor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Methylococcus capsulatus Bath | - |
- |
- |
Purification (Comment) | Organism |
---|---|
stable and active native pMMO from membranes, by solubilization with 1% w/v CHAPS, removal of soluble proteins, gel filtration, anion exchange chromatography, and ultrafiltration | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.034 | - |
purified enzyme, substrates propylene and duroquinol | Methylococcus capsulatus |
0.114 | - |
membrane-bound enzyme, substrates propylene and duroquinol | Methylococcus capsulatus |
0.23 | - |
membrane-bound enzyme, substrates propylene and NADH | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus | methanol + acceptor + H2O | - |
? | |
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus Bath | methanol + acceptor + H2O | - |
? | |
propene + reduced acceptor + H+ + O2 | - |
Methylococcus capsulatus | 1,2-epoxypropane + acceptor + H2O | - |
? | |
propene + reduced acceptor + H+ + O2 | - |
Methylococcus capsulatus Bath | 1,2-epoxypropane + acceptor + H2O | - |
? | |
propylene + duroquinol + O2 | - |
Methylococcus capsulatus | propylene epoxide + duroquinone + H2O | - |
? | |
propylene + duroquinol + O2 | - |
Methylococcus capsulatus Bath | propylene epoxide + duroquinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
particulate methane monooxygenase | - |
Methylococcus capsulatus |
pMMO | - |
Methylococcus capsulatus |
pMMO hydroxylase | - |
Methylococcus capsulatus |
pMMO-H | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Methylococcus capsulatus |