Any feedback?
Literature summary for 1.14.18.3 extracted from
- The particulate methane monooxygenase from Methylococcus capsulatus (Bath) is a novel copper-containing three-subunit enzyme. Isolation and characterization (1998), J. Biol. Chem., 273, 7957-7966.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genetic analysis of pMMO genes | Methylococcus capsulatus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | native occurence of a truncated form of pMMO with different molecular weight | Methylococcus capsulatus |
General Stability
| General Stability | Organism |
|---|---|
| pMMO is very unstable in vitro | Methylococcus capsulatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound particulate enzyme form termed pMMO | Methylococcus capsulatus | 16020 | - |
| membrane | enzyme from cells grown under conditions of high copper availability is membrane-bound | Methylococcus capsulatus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu+ | pMMO, requirement for, contains 12-15 Cu+ ions per molecule of enzyme | Methylococcus capsulatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
| 26000 | - |
1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
| 45000 | - |
1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
| 94000 | - |
pMMO | Methylococcus capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylococcus capsulatus | - |
Bath | - |
| Methylococcus capsulatus | - |
enzyme form pMMO is copper-inducible | - |
| Methylococcus capsulatus Bath | - |
Bath | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to O2, 3 kinetically distinct froms of pMMO with respect to O2 tension, type I is stable with moderate activity, type II is highly unstable to oxygen, type III is an intermediate form | Methylococcus capsulatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pMMO after induction with copper, kinetic type I with respect to O2-sensitivity | Methylococcus capsulatus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, pMMO kinetically type I with respect to O2-sensitivity, repeated freeze-thaw-cycles, stable | Methylococcus capsulatus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
or below: pMMO is irreversibly inactivated | Methylococcus capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Methylococcus capsulatus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
