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Literature summary for 1.14.18.2 extracted from

  • Schlenzka, W.; Shaw, L.; Schauer, R.
    Catalytic properties of the CMP-N-acetylneuraminic acid hydroxylase from the starfish Asterias rubens: comparison with the mammalian enzyme (1993), Biochim. Biophys. Acta, 1161, 131-138.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate activates Asterias rubens
dithiothreitol activates Asterias rubens
glutathione activates Asterias rubens
additional information no activation by non-ionic detergents Asterias rubens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Asterias rubens
azide
-
Asterias rubens
Ca2+ 0.5 mM, 12% inhibition Asterias rubens
CHAPS 15 mM, complete inhibition Asterias rubens
cholic acid 10 mM, 89% inhibition Asterias rubens
decylglucopyranoside 5 mM, 22% inhibition Asterias rubens
ferrozine
-
Asterias rubens
Hg2+ slight inhibition Asterias rubens
KCN
-
Asterias rubens
Mn2+ 0.5 mM, slight inhibition Asterias rubens
additional information not inhibited by increased ionic strength, no inhibition by 1 M NaCl Asterias rubens
octylglucopyranoside 30 mM, 48% inhibition Asterias rubens
Tiron
-
Asterias rubens
Zwittergent 3-12 5 mM, 40% inhibition Asterias rubens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
CMP-N-acetylneuraminate 25°C Asterias rubens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains a non-haem iron cofactor Asterias rubens

Organism

Organism UniProt Comment Textmining
Asterias rubens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
alimentary canal
-
Asterias rubens
-
body wall
-
Asterias rubens
-
gonad
-
Asterias rubens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + NADH + O2
-
Asterias rubens CMP-N-glycoloylneuraminate + NAD+ + H2O
-
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 33
-
Asterias rubens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 43 15°C: about 55% of maximal activity, 43°C: about 30% of maximal activity Asterias rubens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.4
-
Asterias rubens

pH Range

pH Minimum pH Maximum Comment Organism
5.6 6.8 about 60% of maximal activity at pH 5.6 and pH 6.8 Asterias rubens

Cofactor

Cofactor Comment Organism Structure
NADH most effective cofactor, optimal activity at 0.4 mM NADH, higher concentrations are slightly inhibitory Asterias rubens