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Literature summary for 1.14.18.11 extracted from
- Plant sterol biosynthesis: identification of two distinct families of sterol 4alpha-methyl oxidases (2004), Biochem. J., 378, 889-898.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Nicotiana tabacum |
| DNA and amino acid sequence determination and analysis, expression in enzyme-lacking Saccharomyces cerevisiae strain erg25 | Arabidopsis thaliana |
| DNA and amino acid sequence determination and analysis, in vitro transcription | Nicotiana benthamiana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | virus-iduced gene silencing approach for reduction of enzyme expression, sterol analysis of transgenic plants | Nicotiana benthamiana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | non-heme iron | Arabidopsis thaliana |  |
| Iron | non-heme iron | Nicotiana benthamiana | |
| Iron | non-heme iron | Nicotiana tabacum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| additional information | Nicotiana tabacum | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| additional information | Nicotiana benthamiana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8VWZ8 | isozyme SMO2-2; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
| Arabidopsis thaliana | Q9ZW22 | isozyme SMO2-1; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
| Nicotiana benthamiana | Q5J954 | isozyme SMO2; 2 isozymes SMO1 and SMO2 | - |
| Nicotiana tabacum | Q6YAU6 | isozyme SMO2; cv BY2, isozymes SMO1 and SMO2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Arabidopsis thaliana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana benthamiana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana tabacum | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Arabidopsis thaliana | ? | - |
? | |
| additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Nicotiana tabacum | ? | - |
? | |
| additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | Nicotiana benthamiana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Arabidopsis thaliana |
| More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana benthamiana |
| More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4alpha-methyl oxidase | - |
Arabidopsis thaliana |
| 4alpha-methyl oxidase | - |
Nicotiana benthamiana |
| 4alpha-methyl oxidase | - |
Nicotiana tabacum |
| SMO2 | - |
Nicotiana benthamiana |
| SMO2 | - |
Nicotiana tabacum |
| SMO2-1 | - |
Arabidopsis thaliana |
| SMO2-2 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)H | - |
Arabidopsis thaliana | |
| NAD(P)H | - |
Nicotiana benthamiana | |
| NAD(P)H | - |
Nicotiana tabacum | |
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Release 2023.1 (January 2023)
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