Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Nicotiana tabacum |
DNA and amino acid sequence determination and analysis, expression in enzyme-lacking Saccharomyces cerevisiae strain erg25 | Arabidopsis thaliana |
DNA and amino acid sequence determination and analysis, in vitro transcription | Nicotiana benthamiana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | virus-iduced gene silencing approach for reduction of enzyme expression, sterol analysis of transgenic plants | Nicotiana benthamiana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | non-heme iron | Arabidopsis thaliana | |
Iron | non-heme iron | Nicotiana benthamiana | |
Iron | non-heme iron | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
additional information | Nicotiana tabacum | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
additional information | Nicotiana benthamiana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VWZ8 | isozyme SMO2-2; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
Arabidopsis thaliana | Q9ZW22 | isozyme SMO2-1; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
Nicotiana benthamiana | Q5J954 | isozyme SMO2; 2 isozymes SMO1 and SMO2 | - |
Nicotiana tabacum | Q6YAU6 | isozyme SMO2; cv BY2, isozymes SMO1 and SMO2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Arabidopsis thaliana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana benthamiana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana tabacum | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Arabidopsis thaliana | ? | - |
? | |
additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Nicotiana tabacum | ? | - |
? | |
additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | Nicotiana benthamiana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Arabidopsis thaliana |
More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana benthamiana |
More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
4alpha-methyl oxidase | - |
Arabidopsis thaliana |
4alpha-methyl oxidase | - |
Nicotiana benthamiana |
4alpha-methyl oxidase | - |
Nicotiana tabacum |
SMO2 | - |
Nicotiana benthamiana |
SMO2 | - |
Nicotiana tabacum |
SMO2-1 | - |
Arabidopsis thaliana |
SMO2-2 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Arabidopsis thaliana | |
NAD(P)H | - |
Nicotiana benthamiana | |
NAD(P)H | - |
Nicotiana tabacum |