Singh, A.; Jindal, S.; Longchar, B.; Khan, F.; Gupta, V.
Overexpression of Artemisia annua sterol C-4 methyl oxidase gene, AaSMO1, enhances total sterols and improves tolerance to dehydration stress in tobacco (2015), Plant Cell Tissue Organ Cult., 121, 167-181 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expressed in Nicotiana tabacum cultivar Xanthi leaves |
Artemisia annua |
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
endoplasmic reticulum |
- |
Artemisia annua |
5783 |
- |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ |
Artemisia annua |
overall reaction |
3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Artemisia annua |
A0A0A6YZ36 |
variant CIM-Arogya |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ |
overall reaction |
Artemisia annua |
3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 35440, calculated from amino acid sequence |
Artemisia annua |
Synonyms
Synonyms |
Comment |
Organism |
SMO1 |
- |
Artemisia annua |
sterol C-4 methyl oxidase |
- |
Artemisia annua |
sterol-4alpha-methyl-oxidase |
- |
Artemisia annua |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
cytochrome b5 |
- |
Artemisia annua |
|
pI Value
Organism |
Comment |
pI Value Maximum |
pI Value |
Artemisia annua |
calculated from amino acid sequence |
- |
7.8 |
Expression
Organism |
Comment |
Expression |
Artemisia annua |
enzyme expression significantly gets down-regulated (up to about 5fold) under salinity conditions with 400 mM NaCl. The enzyme expression is clearly down-regulated after 3 h treatment with 150 mg/ml chitosan or after 6 h treatment with 0.3 mM methyl jasmonate or after 1.5 h treatment with 1 mM salicylic acid or after 1.5 h treatment with 1 mM trans-cinnamic acid |
down |
Artemisia annua |
transcript levels of the enzyme are consistently increased upon 0.01 mM abscisic acid or 0.1 mM gibberellic acid treatment and induced to more than 2fold relative to the control seedlings. Osmotic/dehydration stress with 400 mM mannitol markedly induces the transcript levels during 6-9 h of treatment and decreases then after |
up |
General Information
General Information |
Comment |
Organism |
physiological function |
enzyme overexpression enhances total sterols and improves tolerance to osmotic/dehydration stress in transgenic tobacco plants |
Artemisia annua |