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Literature summary for 1.14.18.1 extracted from

  • Ionita, E.; Gurgu, L.; Aprodu, I.; Stanciuc, N.; Dalmadi, I.; Bahrim, G.; Rapeanu, G.
    Characterization, purification, and temperature/pressure stability of polyphenol oxidase extracted from plums (Prunus domestica) (2017), Process Biochem., 56, 177-185 .
No PubMed abstract available
Search for more literature for 1.14.18.1

General Stability

General Stability Organism
PPOextracted from plum shows stability at high pressure, with enzyme activation at 500 MPa Prunus domestica

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid 30% inhibition at 0.5 mM, complete inhibition at 5 mM Prunus domestica
Citric acid 24% inhibition at 0.5 mM, 32% inhibition at 5 mM Prunus domestica
EDTA 11% inhibition at 0.5 mM, 15% inhibition at 5 mM Prunus domestica
glycine 9% inhibition at 0.5 mM, 12% inhibition at 5 mM Prunus domestica
L-cysteine 13% inhibition at 0.5 mM, 38% inhibition at 5 mM Prunus domestica
additional information enzyme-inhibitor binding structure and inhibition mechanism, overview Prunus domestica
oxalic acid 16% inhibition at 0.5 mM, 31% inhibition at 5 mM Prunus domestica
sodium chloride 10% inhibition at 0.5 mM, 12% inhibition at 5 mM Prunus domestica
Sodium metabisulfite 13% inhibition at 0.5 mM, 38% inhibition at 5 mM Prunus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 4-methylcatechol + O2 Prunus domestica
-
 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 Prunus domestica
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
2 L-dopa + O2 Prunus domestica
-
 2 dopaquinone + 2 H2O
-
 ?
catechin + O2 Prunus domestica
-
 ? + 2 H2O
-
 ?
chlorogenic acid + O2 Prunus domestica
-
 ? + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Prunus domestica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 32.81fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography Prunus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-methylcatechol + O2
-
 Prunus domestica 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2
-
 Prunus domestica 2 1,2-benzoquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Prunus domestica 2 dopaquinone + 2 H2O
-
 ?
catechin + O2
-
 Prunus domestica ? + 2 H2O
-
 ?
chlorogenic acid + O2
-
 Prunus domestica ? + 2 H2O
-
 ?
additional information the enzyme shows no activity with caffeic acid, ferulic acid, 4-coumaric acid, p-cresol, and L-tyrosine Prunus domestica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Prunus domestica

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Prunus domestica
PPO
-
 Prunus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Prunus domestica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the thermal inactivation follows a first-order kinetic model, with activation energy of Ea150.46 kJ/mol Prunus domestica
25 65 purified enzyme, high stability in the pH range Prunus domestica
60 85 enzyme inactivation kinetics, overview Prunus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Prunus domestica

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 over 50% activity within this range, reduced activity above and below Prunus domestica

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 purified enzyme, high stability in the pH range Prunus domestica