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Literature summary for 1.14.18.1 extracted from

  • Bin, B.H.; Bhin, J.; Yang, S.H.; Shin, M.; Nam, Y.J.; Choi, D.H.; Shin, D.W.; Lee, A.Y.; Hwang, D.; Cho, E.G.; Lee, T.R.
    Membrane-associated transporter protein (MATP) regulates melanosomal pH and influences tyrosinase activity (2015), PLoS ONE, 10, e0129273 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.18.1

Activating Compound

Activating Compound Comment Organism Structure
membrane-associated transporter protein MATP, encoded by gene SLC45A2, regulates melanosomal pH and influences tyrosinase activity, mechanism modeling, overview. The SLC45A2 transcript is highly expressed in human melanoma cells and primary melanocyte. Knockdown of MATP using siRNAs reduces melanin content and tyrosinase activity without any morphological change in melanosomes or the expression of melanogenesis-related proteins, and lowers the the melanosomal pH. The reduced tyrosinase activity in MATP-knockdown cells is due to improper copper binding to tyrosinase under the acidic conditions in melanosomes and that the acidic conditions are due to MATP deletion Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
melanosome
-
 Homo sapiens 42470
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper-containing enzyme, copper is an important element for tyrosinase activity, binding to tyrosinase depends on pH in melanosomes Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-dopa + O2 Homo sapiens
-
 2 dopaquinone + 2 H2O
-
 ?
tyrosine + O2 Homo sapiens
-
 dopaquinone + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation is an important factor for tyrosinase activity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
eye
-
 Homo sapiens
-
hair
-
 Homo sapiens
-
melanocyte primary Homo sapiens
-
melanoma cell
-
 Homo sapiens
-
MNT-1 cell
-
 Homo sapiens
-
skin
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-dopa + O2
-
 Homo sapiens 2 dopaquinone + 2 H2O
-
 ?
tyrosine + O2
-
 Homo sapiens dopaquinone + H2O
-
 ?

Synonyms

Synonyms Comment Organism
L-DOPA oxidase
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction the reduction of tyrosinase activity associated with the knockdown of membrane-associated transporter protein, MATP, using siRNA is readily recovered by copper treatment in the in vitro L-DOPA oxidase activity assay of tyrosinase. N-glycosylation is an important factor for tyrosinase activity, which was reduced in the MATP-knockdown cells Homo sapiens
physiological function membrane-associated transporter protein, MATP, may play an important role in regulating tyrosinase activity via controlling melanosomal pH Homo sapiens