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Literature summary for 1.14.18.1 extracted from

  • Fogal, S.; Carotti, M.; Giaretta, L.; Lanciai, F.; Nogara, L.; Bubacco, L.; Bergantino, E.
    Human tyrosinase produced in insect cells a landmark for the screening of new drugs addressing its activity (2015), Mol. Biotechnol., 57, 45-57 .
    View publication on PubMed
Search for more literature for 1.14.18.1

Cloned(Commentary)

Cloned (Comment) Organism
the wild-type sequence coding for the enzyme is obtained by restriction digestion of the entire cDNA sequence, subcloning in Escherichia coli H10Bac, and recombinant expression of His-tagged enzyme in Spdoptera frugiperda Sf9 cells via the baculovirus transfection method Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 L-tyrosine recombinant enzyme, pH 7.2, 37°C Homo sapiens
0.34
-
 L-Dopa recombinant enzyme, pH 7.2, 37°C Homo sapiens
0.6
-
 4-tert-butylcatechol recombinant enzyme, pH 7.2, 37°C Homo sapiens
0.65
-
 4-tert-butylcatechol recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
1.25
-
 3-(3,5-dihydroxyphenyl)-1-propanoic acid recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
1.5
-
 L-Dopa recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
1.9
-
 L-dopa methyl ester recombinant enzyme, pH 7.2, 37°C Homo sapiens
2.5
-
 4-methylcatechol recombinant enzyme, pH 7.2, 37°C Homo sapiens
4.2
-
 L-dopa methyl ester recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
4.5
-
 catechol recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
5.1
-
 L-tyrosine recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
11
-
 3,4-dihydroxyphenylacetic acid recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
16
-
 dopamine recombinant enzyme, pH 7.2, 37°C Homo sapiens
19
-
 3,4-dihydroxyphenylacetic acid recombinant enzyme, pH 7.2, 37°C Homo sapiens
32
-
 catechol recombinant enzyme, pH 7.2, 37°C Homo sapiens
50
-
 dopamine recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
58
-
 L-isoproterenol recombinant enzyme, pH 7.2, 37°C Homo sapiens
230
-
 L-isoproterenol recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a transmembrane enzyme Homo sapiens 16020
-
additional information the enzyme sequence contains a melanosome-directing signal sequence Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper-containing enzyme, two Cu2+ ions, CuA and CuB Homo sapiens
Cu2+ a copper-containing enzyme, two Cu2+ ions, CuA and CuB Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-dopa + O2 Homo sapiens
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Streptomyces antibioticus
-
 2 dopaquinone + 2 H2O
-
 ?
L-tyrosine + O2 Homo sapiens
-
 dopaquinone + H2O
-
 ?
L-tyrosine + O2 Streptomyces antibioticus
-
 dopaquinone + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14679
-
-
Streptomyces antibioticus P07524
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Spdoptera frugiperda Sf9 cells by nickel affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-dopa + O2
-
 Homo sapiens 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Streptomyces antibioticus 2 dopaquinone + 2 H2O
-
 ?
3,4-dihydroxyphenylacetic acid + O2
-
 Homo sapiens ?
-
 ?
3,4-dihydroxyphenylacetic acid + O2
-
 Streptomyces antibioticus ?
-
 ?
3-(3,5-dihydroxyphenyl)-1-propanoic acid + O2 low activity Streptomyces antibioticus ?
-
 ?
4-methylcatechol + O2
-
 Homo sapiens ?
-
 ?
4-methylcatechol + O2
-
 Streptomyces antibioticus ?
-
 ?
4-tert-butylcatechol + O2
-
 Homo sapiens ?
-
 ?
4-tert-butylcatechol + O2
-
 Streptomyces antibioticus ?
-
 ?
catechol + O2
-
 Homo sapiens ?
-
 ?
catechol + O2
-
 Streptomyces antibioticus ?
-
 ?
dopamine + O2
-
 Homo sapiens ?
-
 ?
dopamine + O2
-
 Streptomyces antibioticus ?
-
 ?
L-dopa methyl ester + O2
-
 Homo sapiens ?
-
 ?
L-dopa methyl ester + O2
-
 Streptomyces antibioticus ?
-
 ?
L-isoproterenol + O2
-
 Homo sapiens ?
-
 ?
L-isoproterenol + O2
-
 Streptomyces antibioticus ?
-
 ?
L-tyrosine + O2
-
 Homo sapiens dopaquinone + H2O
-
 ?
L-tyrosine + O2
-
 Streptomyces antibioticus dopaquinone + H2O
-
 ?
additional information no activity with [3-(3,5-dihydroxyphenyl)-1-propanoic acid] Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66000 + x * 57000, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Streptomyces antibioticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.28
-
 L-isoproterenol recombinant enzyme, pH 7.2, 37°C Homo sapiens
2.6
-
 L-tyrosine recombinant enzyme, pH 7.2, 37°C Homo sapiens
3
-
 3-(3,5-dihydroxyphenyl)-1-propanoic acid recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
5.8
-
 L-isoproterenol recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
5.9
-
 3,4-dihydroxyphenylacetic acid recombinant enzyme, pH 7.2, 37°C Homo sapiens
7.9
-
 L-tyrosine recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
12
-
 4-methylcatechol recombinant enzyme, pH 7.2, 37°C Homo sapiens
20.5
-
 4-tert-butylcatechol recombinant enzyme, pH 7.2, 37°C Homo sapiens
38.1
-
 L-Dopa recombinant enzyme, pH 7.2, 37°C Homo sapiens
44
-
 catechol recombinant enzyme, pH 7.2, 37°C Homo sapiens
57
-
 L-dopa methyl ester recombinant enzyme, pH 7.2, 37°C Homo sapiens
61
-
 dopamine recombinant enzyme, pH 7.2, 37°C Homo sapiens
80
-
 catechol recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
113
-
 3,4-dihydroxyphenylacetic acid recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
187
-
 L-dopa methyl ester recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
197
-
 L-Dopa recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
210
-
 dopamine recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus
1650
-
 4-tert-butylcatechol recombinant enzyme, pH 7.2, 37°C Streptomyces antibioticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens
7.2
-
 assay at Streptomyces antibioticus

General Information

General Information Comment Organism
physiological function human tyrosinase is the first enzyme of the multistep process of melanogenesis. It catalyzes the hydroxylation of L-tyrosine to L-dihydroxyphenylalanine and the following oxidation of o-diphenol to the corresponding quinone, L-dopaquinone Homo sapiens
physiological function tyrosinase is the first enzyme of the multistep process of melanogenesis. It catalyzes the hydroxylation of L-tyrosine to L-dihydroxyphenylalanine and the following oxidation of o-diphenol to the corresponding quinone, L-dopaquinone Streptomyces antibioticus