Application | Comment | Organism |
---|---|---|
additional information | when applied in various cross-linking reactions the tyrosinase is able to cross-link casein and gelatin in the absence of a phenolic compound, showing potential for application in the food industry and for the production of biomaterials | Streptomyces pharetrae |
additional information | when applied in various cross-linking reactions the tyrosinase is able to cross-link casein and gelatine in the absence of a phenolic compound, showing potential forapplication in the food industry and for the production of biomaterials | Streptomyces polyantibioticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arbutin | - |
Streptomyces pharetrae | |
arbutin | - |
Streptomyces polyantibioticus | |
Co2+ | 22% inhibition at 5 mM | Streptomyces polyantibioticus | |
Cu2+ | over 60% inhibition at 5 mM | Streptomyces pharetrae | |
Cu2+ | over 60% inhibition at 5 mM | Streptomyces polyantibioticus | |
EDTA | - |
Streptomyces pharetrae | |
EDTA | - |
Streptomyces polyantibioticus | |
Fe2+ | complete inhibition at 5 mM | Streptomyces pharetrae | |
additional information | the enzyme is strongly inhibited by the reducing agents | Streptomyces pharetrae | |
additional information | the enzyme is strongly inhibited by the reducing agents | Streptomyces polyantibioticus | |
sodium chloride | - |
Streptomyces pharetrae | |
sodium chloride | - |
Streptomyces polyantibioticus | |
sodium dodecylsulfate | - |
Streptomyces pharetrae | |
sodium dodecylsulfate | - |
Streptomyces polyantibioticus | |
Zn2+ | 70% inhibition at 5 mM | Streptomyces pharetrae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at 5 mM | Streptomyces pharetrae | |
Cu2+ | a copper-containing enzyme | Streptomyces pharetrae | |
Cu2+ | a copper-containing enzyme | Streptomyces polyantibioticus | |
Mg2+ | activates at 5 mM | Streptomyces pharetrae | |
Mn2+ | activates at 5 mM | Streptomyces pharetrae | |
additional information | no effect by Co2+ at 5 mM | Streptomyces pharetrae | |
additional information | no effect by Zn2+ and Fe2+ at 5 mM | Streptomyces polyantibioticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | Streptomyces pharetrae | - |
2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | Streptomyces polyantibioticus | - |
2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | Streptomyces polyantibioticus SPR | - |
2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | Streptomyces pharetrae CZA14 | - |
2 dopaquinone + 2 H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 10% activity remaining at 40% v/v, 0% at 50% v/v | Streptomyces pharetrae |
2-propanol | 70% activity remaining at 30% v/v, below 5% at 40% v/v | Streptomyces polyantibioticus |
Acetone | 20% activity remaining at 45% v/v, 0% at 50% v/v | Streptomyces polyantibioticus |
Acetone | 75% activity remaining at 45% v/v, 18% at 50% v/v | Streptomyces pharetrae |
acetonitrile | 10% activity remaining at 30% v/v, 0% at 40% v/v | Streptomyces pharetrae |
acetonitrile | 70% activity remaining at 20% v/v, 0% at 30% v/v | Streptomyces polyantibioticus |
DMSO | 80% activity remaining at 40% v/v, 65% at 50% v/v | Streptomyces pharetrae |
DMSO | over 85% activity remaining at 40% v/v, 65% at 50% v/v | Streptomyces polyantibioticus |
Ethanol | 65% activity remaining at 40% v/v, 15% at 50% v/v | Streptomyces pharetrae |
Ethanol | 80% activity remaining at 30% v/v, 0% at 40% v/v | Streptomyces polyantibioticus |
Methanol | 20% activity remaining at 40% v/v, 0% at 50% v/v | Streptomyces polyantibioticus |
Methanol | 70% activity remaining at 40% v/v, 18% at 50% v/v | Streptomyces pharetrae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces pharetrae | - |
- |
- |
Streptomyces pharetrae CZA14 | - |
- |
- |
Streptomyces polyantibioticus | - |
- |
- |
Streptomyces polyantibioticus SPR | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 11.84fold by ammonium sulfate fractionation, dialysis, and separation by additin of 20% (w/v) PEG 8000 solution, followed by anion exchange chromatography | Streptomyces polyantibioticus |
native enzyme 5.16fold by ammonium sulfate fractionation, dialysis, and separation by additin of 20% (w/v) PEG 8000 solution, followed by anion exchange chromatography | Streptomyces pharetrae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimizatin of tyrosinase production for industrial applications, method optimization, overview | Streptomyces pharetrae | - |
additional information | optimizatin of tyrosinase production for industrial applications, method optimization, overview | Streptomyces polyantibioticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.07 | - |
purified enzyme, pH 6.5, 22°C | Streptomyces pharetrae |
23.8 | - |
purified enzyme, pH 6.5, 22°C | Streptomyces polyantibioticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | - |
Streptomyces pharetrae | 2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | - |
Streptomyces polyantibioticus | 2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | - |
Streptomyces polyantibioticus SPR | 2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | - |
Streptomyces pharetrae CZA14 | 2 dopaquinone + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30500, SDS-PAGE | Streptomyces polyantibioticus |
? | x * 32600, SDS-PAGE | Streptomyces pharetrae |
Synonyms | Comment | Organism |
---|---|---|
CZA14Tyr | - |
Streptomyces pharetrae |
melC2 | - |
Streptomyces pharetrae |
melC2 | - |
Streptomyces polyantibioticus |
SPRTyr | - |
Streptomyces polyantibioticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 35 | first optimum | Streptomyces polyantibioticus |
30 | 40 | first optimum | Streptomyces pharetrae |
50 | - |
second optimum | Streptomyces polyantibioticus |
60 | - |
second optimum | Streptomyces pharetrae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
enzyme CZA14Tyr, 1 h, stable up to | Streptomyces pharetrae |
40 | - |
enzyme SPRTyr , 1 h, stable up to | Streptomyces polyantibioticus |
60 | - |
enzyme CZA14Tyr shows residual activity of 29.50% (0.53% for SPRTyr) after 10 min incubation at 60°C, but rapidly loses 97% activity after 20 min of incubation | Streptomyces pharetrae |
60 | - |
enzyme SPRTyr shows residual activity of 0.53% after10 min incubation at 60°C meaning inactivation | Streptomyces polyantibioticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptomyces pharetrae |
6.5 | - |
- |
Streptomyces polyantibioticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | enzyme SPRTyr, stable at | Streptomyces polyantibioticus |
6.5 | 10 | enzyme CZA14Tyr, stable at | Streptomyces pharetrae |