Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-dimethylimidazolium methylsulfate | 69.7% residual activity at 5% (w/v) | Agaricus bisporus | |
1-butyl-3-methylimidazolium methylsulfate | 47.8% residual activity at 5% (w/v) | Agaricus bisporus | |
1-ethyl-3-methylimidazolium methylsulfate | 64.1% residual activity at 5% (w/v) | Agaricus bisporus | |
acetone | increasing solvent concentration up to 80% (v/v) yields a gradual reduction in the activity of the soluble and cross-linked enzyme forms, the cross-linked enzyme aggregate shows about 40% residual activity after incubation in acetone for about 34 h | Agaricus bisporus | |
chloroform | the cross-linked enzyme aggregate shows about 30% residual activity after incubation in chloroform for about 3 h | Agaricus bisporus | |
choline acetate | 27.9% residual activity at 5% (w/v) | Agaricus bisporus | |
choline dihydrophosphate | 27.4% residual activity at 5% (w/v) | Agaricus bisporus | |
choline methylsulfonate | 39.7% residual activity at 5% (w/v) | Agaricus bisporus | |
choline nitrate | 54.6% residual activity at 5% (w/v) | Agaricus bisporus | |
dioxane | increasing solvent concentration up to 80% (v/v) yields a gradual reduction in the activity of the soluble and cross-linked enzyme forms, the cross-linked enzyme aggregate shows about 40% residual activity after incubation in dioxane for about 62 h | Agaricus bisporus | |
EDTA | - |
Agaricus bisporus | |
Guanidine-HCl | treatment with guanidine-HCl at increasing concentrations (0-800 mM) results in a reduced activity for both enzyme forms, but aggregation as cross-linked enzyme aggregate improves tyrosinase stability at higher concentrations (above 314 mM) | Agaricus bisporus | |
hexane | the cross-linked enzyme aggregate shows about 20% residual activity after incubation in hexane for about 24 h | Agaricus bisporus | |
methanol | the soluble enzyme retains 7.8% of its original activity, as compared to 31% by the cross-linked enzyme aggregates, after being incubated in the presence of 40% (v/v) methanol | Agaricus bisporus | |
NaN3 | treatment with NaN3 at increasing concentrations (0-4 mM) results in a reduced activity for both soluble and cross-linked enzyme forms, but aggregation as cross-linked enzyme aggregates improves tyrosinase stability at higher concentrations (above 0.4 mM) | Agaricus bisporus | |
SDS | a sharp decrease in the activity of the soluble enzyme is noted from 0 to 17 mM SDS | Agaricus bisporus | |
tert-butanol | the cross-linked enzyme aggregate shows about 50% residual activity after incubation in tert-butanol for about 326 h | Agaricus bisporus | |
tetrabutylammonium acetate | 24.1% residual activity at 5% (w/v) | Agaricus bisporus | |
tetrabutylammonium methylsulfonate | 45.3% residual activity at 5% (w/v) | Agaricus bisporus | |
tetramethylammonium acetate | 30% residual activity at 5% (w/v) | Agaricus bisporus | |
tributylammonium dihydrophosphate | 27.5% residual activity at 5% (w/v) | Agaricus bisporus | |
triethylammonium dihydrophosphate | 23.4% residual activity at 5% (w/v) | Agaricus bisporus | |
trimethylammonium dihydrophosphate | 12% residual activity at 5% (w/v) | Agaricus bisporus | |
trimethylammonium methylsulfonate | 40.9% residual activity at 5% (w/v) | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
L-Dopa | soluble enzyme, pH 6.0, 55°C | Agaricus bisporus | |
1.16 | - |
L-Dopa | cross-linked enzyme aggregate, pH 6.0, 30°C | Agaricus bisporus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme contains copper | Agaricus bisporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-DOPA + O2 | - |
Agaricus bisporus | dopaquinone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tyrosinase | - |
Agaricus bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | cross-linked enzyme aggregate | Agaricus bisporus |
55 | - |
soluble enzyme | Agaricus bisporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the soluble enzyme shows half-lives of about 120 min, 30 min, and 10 min at 40, 50, and 60°C, respectively. The cross-linked enzyme aggregates show half-lives of about 320 min, 120 min, and 30 min at 40, 50, and 60°C, respectively | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Agaricus bisporus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | the soluble enzyme shows half-lives of about 11 min, 35 min, and 3 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively. The cross-linked enzyme aggregates show half-lives of about 11 min, 35 min, and 1 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively | Agaricus bisporus |