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Literature summary for 1.14.18.1 extracted from
- Purification and characterisation of a polyphenol oxidase from Boletus erythropus and investigation of its catalytic efficiency in selected organic solvents (2010), Food Chem., 119, 1044-1049.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | - |
Neoboletus erythropus |  |
| benzoic acid | - |
Neoboletus erythropus | |
| Ca2+ | 74.2% residual activity at 1 mM | Neoboletus erythropus | |
| Cu2+ | 88.3% residual activity at 1 mM | Neoboletus erythropus | |
| Li+ | 72.5% residual activity at 1 mM | Neoboletus erythropus | |
| Mn2+ | 62.9% residual activity at 1 mM | Neoboletus erythropus | |
| Ni2+ | 56.6% residual activity at 1 mM | Neoboletus erythropus | |
| Sodium azide | - |
Neoboletus erythropus | |
| Sodium metabisulfite | - |
Neoboletus erythropus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
4-methylcatechol | pH 8.0, 20°C | Neoboletus erythropus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 127.3% activity at 1 mM | Neoboletus erythropus | |
| additional information | the enzyme is not influenced by Zn2+, Cr2+, Na+, K+, Mg2+, and Cd2+ | Neoboletus erythropus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Neoboletus erythropus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neoboletus erythropus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity column chromatography, gel filtration | Neoboletus erythropus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylcatechol + O2 | highest activity | Neoboletus erythropus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| L-DOPA + O2 | - |
Neoboletus erythropus | dopaquinone + H2O | - |
? | |
| L-tyrosine + O2 | - |
Neoboletus erythropus | L-DOPA + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyphenol oxidase | - |
Neoboletus erythropus |
| PPO | - |
Neoboletus erythropus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
using 4-methylcatechol as a substrate | Neoboletus erythropus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 80 | the enzyme is stable between 10 and 30°C after 24 h incubation, The enzyme activity declines as the temperature increases but the enzyme is not completely inactivated even at 80°C | Neoboletus erythropus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
using 4-methylcatechol as a substrate | Neoboletus erythropus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | the enzyme is extremely stable between pH 3.0 and 9.0 after 24 h incubation at 4°C retaining at least 85% of its original activity | Neoboletus erythropus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
pH 8.0, 20°C | Neoboletus erythropus | Sodium metabisulfite | |
| 0.08 | - |
pH 8.0, 20°C | Neoboletus erythropus | ascorbic acid | |
| 10 | - |
pH 8.0, 20°C | Neoboletus erythropus | Sodium azide | |
| 10.2 | - |
pH 8.0, 20°C | Neoboletus erythropus | benzoic acid | |
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