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Literature summary for 1.14.18.1 extracted from
- Kinetic characterization of diphenolase activity from Streptomyces antibioticus tyrosinase in the presence and absence of cyclodextrins (2007), J. Mol. Catal. B, 47, 143-148.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the presence of this enzyme has a large impact in the food industry because it is the main enzyme involved in the undesirable browning of fruits and vegetables during processing and storage | Streptomyces antibioticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | reducing agent | Streptomyces antibioticus |  |
| Cinnamic acid | substrate analogue | Streptomyces antibioticus | |
| Hexestrol | best inhibitors are tropolone, hinokitiol and hexestrol | Streptomyces antibioticus | |
| hinokitiol | best inhibitors are tropolone, hinokitiol and hexestrol | Streptomyces antibioticus | |
| L-cysteine | reducing agent | Streptomyces antibioticus | |
| L-mimosine | substrate analogue | Streptomyces antibioticus | |
| Metabisulfite | reducing agent | Streptomyces antibioticus | |
| additional information | natural occurring compounds like cyclodextrins (two different types of cyclodextrins (OH-beta-CDs and gamma-CDs) tested on enzymatic diphenolic oxidation), which are used in food technology and in pharmacology, can release the inhibitory effect of the inhibitors | Streptomyces antibioticus | |
| tropolone | substrate analogue, best inhibitors are tropolone, hinokitiol and hexestrol | Streptomyces antibioticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
tert-butylcathechol | - |
Streptomyces antibioticus | |
| 8.4 | - |
dopamine | - |
Streptomyces antibioticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces antibioticus | - |
gram-positive soil bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification by PEG-8000/phosphate phase partitioning and ammonium sulfate fractionation using tert-butylcathechol (TBC) and dopamine | Streptomyces antibioticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dopamine + 1/2 O2 | hydrophilic substrate | Streptomyces antibioticus | dopamine quinone + H2O | - |
? | |
| tert-butylcatechol + 1/2 O2 | hydrophobic substrate | Streptomyces antibioticus | 4-(tert-butyl)benzo-1,2-quinone + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diphenolase | - |
Streptomyces antibioticus |
| monophenol, dihydroxy-L-phenylalanine:oxygen oxidoreductase | - |
Streptomyces antibioticus |
| tyrosinase | - |
Streptomyces antibioticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Streptomyces antibioticus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0042 | - |
tropolone | competitive inhibition | Streptomyces antibioticus | |
| 0.049 | - |
Hexestrol | - |
Streptomyces antibioticus | |
| 0.5 | - |
hinokitiol | - |
Streptomyces antibioticus | |
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