Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | - |
Litchi chinensis | |
glutathione | strong inhibitor even at low concentrations | Litchi chinensis | |
L-cysteine | strong inhibitor even at low concentrations | Litchi chinensis | |
additional information | results indicate that the inhibition mechanisms of thiol groups are different from those of halide salts | Litchi chinensis | |
NaF | inhibits moderately | Litchi chinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
(-)-epicatechin | - |
Litchi chinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Litchi chinensis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pericarp | - |
Litchi chinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-epicatechin + 1/2 O2 | the major endogenous polyphenol in litchi pericarp | Litchi chinensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cresolase | - |
Litchi chinensis |
monophenol monooxygenase | - |
Litchi chinensis |
polyphenol oxidase | - |
Litchi chinensis |
PPO | - |
Litchi chinensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Litchi chinensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 75 | determine optimum temperature and thermostability | Litchi chinensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
from thermostability studies, the Ki value increases with temperature and the results suggest that the enzyme is unstable above 45°C. Above 45°C, the PPO activity decreases with increasing temperature. Strong evidence that the denaturalization temperature of PPO is near 70°C | Litchi chinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the activity of the PPO is highly pH-dependent | Litchi chinensis |
7.5 | - |
optimum pH for PPO activity with (-)-epicatechin as a substrate | Litchi chinensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | measuring effect of pH on PPO activity and stability | Litchi chinensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | enzyme is stable in the pH range of 6.0-8.0 | Litchi chinensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki value increases with temperature and the results suggest that the enzyme is unstable above 45°C | Litchi chinensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.13 | - |
- |
Litchi chinensis | L-cysteine | |
0.19 | - |
- |
Litchi chinensis | glutathione | |
16.84 | - |
- |
Litchi chinensis | NaF |