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Literature summary for 1.14.18.1 extracted from

  • Liu, L.; Cao, S.; Xie, B.; Sun, Z.; Li, X.; Miao, W.
    Characterization of polyphenol oxidase from litchi pericarp using (-)-epicatechin as substrate (2007), J. Agric. Food Chem., 55, 7140-7143.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid
-
Litchi chinensis
glutathione strong inhibitor even at low concentrations Litchi chinensis
L-cysteine strong inhibitor even at low concentrations Litchi chinensis
additional information results indicate that the inhibition mechanisms of thiol groups are different from those of halide salts Litchi chinensis
NaF inhibits moderately Litchi chinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.65
-
(-)-epicatechin
-
Litchi chinensis

Organism

Organism UniProt Comment Textmining
Litchi chinensis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pericarp
-
Litchi chinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-epicatechin + 1/2 O2 the major endogenous polyphenol in litchi pericarp Litchi chinensis ?
-
?

Synonyms

Synonyms Comment Organism
cresolase
-
Litchi chinensis
monophenol monooxygenase
-
Litchi chinensis
polyphenol oxidase
-
Litchi chinensis
PPO
-
Litchi chinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Litchi chinensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 75 determine optimum temperature and thermostability Litchi chinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
from thermostability studies, the Ki value increases with temperature and the results suggest that the enzyme is unstable above 45°C. Above 45°C, the PPO activity decreases with increasing temperature. Strong evidence that the denaturalization temperature of PPO is near 70°C Litchi chinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
the activity of the PPO is highly pH-dependent Litchi chinensis
7.5
-
optimum pH for PPO activity with (-)-epicatechin as a substrate Litchi chinensis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8 measuring effect of pH on PPO activity and stability Litchi chinensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 enzyme is stable in the pH range of 6.0-8.0 Litchi chinensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information Ki value increases with temperature and the results suggest that the enzyme is unstable above 45°C Litchi chinensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.13
-
-
Litchi chinensis L-cysteine
0.19
-
-
Litchi chinensis glutathione
16.84
-
-
Litchi chinensis NaF