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Literature summary for 1.14.18.1 extracted from

  • Kennedy, L.J.; Selvi, P.K.; Padmanabhan, A.; Hema, K.N.; Sekaran, G.
    Immobilization of polyphenol oxidase onto mesoporous activated carbons --isotherm and kinetic studies (2007), Chemosphere, 69, 262-270.
    View publication on PubMed
Search for more literature for 1.14.18.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters for free and immobilized enzymes, determined according to the Michaelis-Menten equation, show a lower Km value for PPO-MAC400 than for the free enzyme, indicating higher affinity towards substrate, while PPO-MAC200 exhibits a higher Km value Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum tuberosum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 PPO is immobilized onto MAC400 and MAC200 at various enzyme activities 50000, 100000, 200000, 300000 U/l, at pH 5-8, and at temperature ranging from 10 to 40°C. The optimum conditions for immobilization of PPO onto MAC400 and MAC200 are temperature 40°C, pH 5 and at PPO activity equal to 300000 U/l Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dopa + 1/2 O2
-
 Solanum tuberosum L-dopaquinone + H2O
-
 ?
L-tyrosine + O2 + AH2
-
 Solanum tuberosum L-dopa + H2O + A
-
 ?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Solanum tuberosum
PPO
-
 Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 the optimum conditions for immobilization of PPO onto MAC400 and MAC200 are temperature 40°C, pH 5 and at PPO activity equal to 300000 U/l Solanum tuberosum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 PPO is immobilized onto MAC400 and MAC200 at various enzyme activities 50000, 100000, 200000, 300000 U/l, at pH 5-8, and at temperature ranging from 10 to 40°C Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 the optimum conditions for immobilization of PPO onto MAC400 and MAC200 are temperature 40°C, pH 5 and at PPO activity equal to 300000 U/l Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
5 8 PPO is immobilized onto MAC400 and MAC200 at various enzyme activities 50000, 100000, 200000, 300000 U/l, at pH 5-8, and at temperature ranging from 10 to 40°C Solanum tuberosum