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Literature summary for 1.14.18.1 extracted from

  • Saiedian, S.; Keyhani, E.; Keyhani, J.
    Polyphenol oxidase activity in dormant saffron (Crocus sativus L.) corm (2007), Acta Physiol. Plant., 29, 463-471.
No PubMed abstract available
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
kojic acid
-
 Crocus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 pyrogallol detected in dormant saffron corms Crocus sativus
2 3 catechol detected in dormant saffron corms Crocus sativus
4.2
-
 L-Dopa detected in dormant saffron corms Crocus sativus
130
-
 p-Cresol detected in dormant saffron corms Crocus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 dormant saffron corm extracts are submitted to non-denaturing polyacrylamide gel electrophoresis, staining for PPO activity revealed three distinct bands 39000, 60000, 15000 Da Crocus sativus
60000
-
 dormant saffron corm extracts are submitted to non-denaturing polyacrylamide gel electrophoresis, staining for PPO activity revealed three distinct bands 39000, 60000, 15000 Da Crocus sativus
150000
-
 dormant saffron corm extracts are submitted to non-denaturing polyacrylamide gel electrophoresis, staining for PPO activity revealed three distinct bands 39000, 60000, 15000 Da Crocus sativus

Organism

Organism UniProt Comment Textmining
Crocus sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of PPO from dormant saffron corms is carried out by ammonium sulfate fractionation (between 30 and 80% ammonium sulfate) followed by dialysis against phosphate buffer 0.1 M, pH 7.00, 0.02% PMSF, then chromatography through CM-Sephadex C-25 equilibrated with the same buffer Crocus sativus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
 L-dopa, detected in dormant saffron corms Crocus sativus
45
-
 pyrogallol, detected in dormant saffron corms Crocus sativus
600
-
 p-cresol, detected in dormant saffron corms Crocus sativus
900
-
 catechol, detected in dormant saffron corms Crocus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + 1/2 O2
-
 Crocus sativus 4-methyl-1,2-benzoquinone + H2O
-
 ?
catechol + 1/2 O2
-
 Crocus sativus 1,2-benzoquinone + H2O
-
 ?
L-dopa + 1/2 O2
-
 Crocus sativus L-dopaquinone + H2O
-
 ?
additional information no activity is detectable with L-tyrosine, tyramine or phenol as substrate Crocus sativus ?
-
 ?
p-cresol + O2 + AH2
-
 Crocus sativus 4-methylcatechol + H2O + A
-
 ?
pyrogallol + 1/2 O2
-
 Crocus sativus ?
-
 ?

Synonyms

Synonyms Comment Organism
monophenol, o-diphenol:O2 oxidoreductase
-
 Crocus sativus
polyphenol oxidase EC1.14.18.1, EC1.10.3.1 Crocus sativus
PPO
-
 Crocus sativus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 25 assays are conducted at room tempertature Crocus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 two pH optima, respectively at 4.5 and 6.7, are observed with all substrates and a third one, at 8.5, is found with L-dopa and p-cresol Crocus sativus
6.7
-
 two pH optima, respectively at 4.5 and 6.7, are observed with all substrates and a third one, at 8.5, is found with L-dopa and p-cresol Crocus sativus
8.5
-
 two pH optima, respectively at 4.5 and 6.7, are observed with all substrates and a third one, at 8.5, is found with L-dopa and p-cresol Crocus sativus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.22
-
 with catechol as substrate, competitive inhibition Crocus sativus kojic acid
0.35
-
 with pyrogallol as substrate, mixed inhibition Crocus sativus kojic acid
0.5
-
 with p-cresol as substrate, uncompetitive inhibition Crocus sativus kojic acid
0.7
-
 with L-dopa as substrate, noncompetitive inhibition Crocus sativus kojic acid