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Literature summary for 1.14.18.1 extracted from

  • Guelcin, I.; Kuefrevioglu, O.I.; Oktay, M.
    Purification and characterization of polyphenol oxidase from nettle (Urtica dioica L.) and inhibitory effects of some chemicals on enzyme activity (2005), J. Enzyme Inhib. Med. Chem., 20, 297-302.
    View publication on PubMed
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol competitive Urtica dioica
benzoic acid noncompetitive Urtica dioica
glutathione competitive Urtica dioica
L-ascorbic acid competitive Urtica dioica
L-cysteine chloride competitive Urtica dioica
salicylic acid uncompetitive Urtica dioica
Sodium azide noncompetitive Urtica dioica
Sodium cyanide noncompetitive Urtica dioica
Sodium diethyl dithiocarbamate cstrong competitive inhibitor Urtica dioica
Sodium metabisulfite competitive Urtica dioica
Thiourea competitive Urtica dioica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
 trans-cinnamic acid pH 3.5, 25°C Urtica dioica
0.79
-
 L-tyrosine pH 4.5, 25°C Urtica dioica
21.4
-
 L-Dopa pH 3.5, 25°C Urtica dioica

Organism

Organism UniProt Comment Textmining
Urtica dioica
-
 two isozymes
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography Urtica dioica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.57
-
 purified enzyme Urtica dioica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-cresol + O2
-
 Urtica dioica ?
-
 ?
L-tyrosine + L-dopa + O2 high activity, best substrate Urtica dioica L-dopa + dopaquinone + H2O
-
 ?
additional information substrate specificity, overview, activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview Urtica dioica ?
-
 ?
trans-cinnamic acid + O2
-
 Urtica dioica ?
-
 ?

Synonyms

Synonyms Comment Organism
More cf. EC 1.10.3.1 Urtica dioica
polyphenol oxidase
-
 Urtica dioica
PPO
-
 Urtica dioica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 65 dependent on the substrate, overview Urtica dioica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 80
-
 Urtica dioica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 purified enzyme, complete inactivation within 3 min Urtica dioica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4.5 dependent on the substrate, overview Urtica dioica

pH Range

pH Minimum pH Maximum Comment Organism
3 5.5
-
 Urtica dioica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000179
-
 Sodium diethyl dithiocarbamate pH 6.5, 35°C, versus L-tyrosine Urtica dioica
0.00137
-
 L-cysteine chloride pH 6.5, 35°C, versus L-tyrosine Urtica dioica
0.0131
-
 benzoic acid pH 6.5, 35°C Urtica dioica
0.0164
-
 Sodium metabisulfite pH 6.5, 35°C, versus L-tyrosine Urtica dioica
0.0168
-
 L-ascorbic acid pH 6.5, 35°C, versus L-tyrosine Urtica dioica
0.074
-
 Sodium cyanide pH 6.5, 35°C Urtica dioica
0.084
-
 2-mercaptoethanol pH 6.5, 35°C, versus L-tyrosine Urtica dioica
0.213
-
 Thiourea pH 6.5, 35°C, versus L-tyrosine Urtica dioica
0.25
-
 Sodium azide pH 6.5, 35°C Urtica dioica
0.67
-
 salicylic acid pH 6.5, 35°C Urtica dioica