Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.18.1 extracted from

  • Mikkelsen, R.B.; Triplett, E.L.
    Tyrosinases in Rana pipiens. Purification and physical properties (1975), J. Biol. Chem., 250, 638-643.
    View publication on PubMed
Search for more literature for 1.14.18.1

Activating Compound

Activating Compound Comment Organism Structure
Photoactivation
-
 Lithobates pipiens

General Stability

General Stability Organism
trypsin causes no inactivation Lithobates pipiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, isoenzyme 1 and 2, high speed sedimentation equilibrium in presence of SDS Lithobates pipiens
32000
-
 x * 32000, calculated from amino acid analysis Lithobates pipiens
33000
-
 x * 33000, isoenzyme 1 and 2, SDS-PAGE Lithobates pipiens

Organism

Organism UniProt Comment Textmining
Lithobates pipiens
-
 frog, 2 probably identical enzymes
-

Purification (Commentary)

Purification (Comment) Organism
2 probably identical isozymes, ammonium sulfate, DEAE-cellulose, CM-sephadex, Bio-gel P300 Lithobates pipiens

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Lithobates pipiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.3
-
 isoenzyme 2, tyrosine hydroxylation Lithobates pipiens
17.5
-
 isoenzyme 1, tyrosine hydroxylation Lithobates pipiens
69.5
-
 isoenzyme 2, dopa oxidation Lithobates pipiens
72
-
 isoenzyme 1, dopa oxidation Lithobates pipiens

Subunits

Subunits Comment Organism
? x * 32000, calculated from amino acid analysis Lithobates pipiens
? x * 30000, isoenzyme 1 and 2, high speed sedimentation equilibrium in presence of SDS Lithobates pipiens
? x * 33000, isoenzyme 1 and 2, SDS-PAGE Lithobates pipiens