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Literature summary for 1.14.18.1 extracted from

  • Goodenough, P.W.; Kessell, S.; Lea, A.G.H.; Loeffler, T.
    Mono- and diphenolase activity from fruit of Malus pumila (1983), Phytochemistry, 22, 359-363.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
L-Dopa and analogs, activation Malus sp.

Inhibitors

Inhibitors Comment Organism Structure
O2 at concentrations above 30% Malus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 4.3% O2 Malus sp.
0.2
-
caffeic acid
-
Malus sp.
0.2
-
3-hydroxyphloridzin
-
Malus sp.
2.7
-
L-Dopa
-
Malus sp.
3.2
-
4-methylcatechol
-
Malus sp.
4
-
p-coumaric acid
-
Malus sp.
4.7
-
catechin
-
Malus sp.
5.7
-
epicatechin
-
Malus sp.
5.9
-
chlorogenic acid
-
Malus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Malus sp.

Organism

Organism UniProt Comment Textmining
Malus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Malus sp.

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Malus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyphloridzin + O2
-
Malus sp. ?
-
?
4-methylcatechol + O2
-
Malus sp. 4-methyl-o-benzoquinone + H2O
-
?
caffeic acid + 1/2 O2
-
Malus sp. caffeoyl quinone + H2O
-
?
catechin + O2
-
Malus sp. catechin-O-quinone + H2O
-
r
chlorogenic acid + O2
-
Malus sp. chlorogenoquinone + H2O
-
?
D-catechin + O2
-
Malus sp. ?
-
?
epicatechin + O2
-
Malus sp. ?
-
?
L-DOPA + O2
-
Malus sp. dopaquinone + H2O
-
r
L-epicatechin + O2
-
Malus sp. ?
-
?
p-coumaric acid + O2 + AH2
-
Malus sp. caffeic acid + H2O + A
-
?
phloridzin + O2
-
Malus sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Malus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
minimal activity at pH 5.5 Malus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
no activity below Malus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 4.5 optimal stability at pH 4.5, less stable above pH 6.0 Malus sp.
6
-
4 days at 2°C, 70% inactivation Malus sp.