Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.18.1 extracted from

  • Wichers, H.J.; Peetsma, G.J.; Malingre, T.M.; Huizing, H.J.
    Purification and properties of a phenol oxidase derived from suspension cultures of Mucuna pruriens (1984), Planta, 162, 334-341.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate activation of tyrosine hydroxylase activity Mucuna pruriens

General Stability

General Stability Organism
tyrosine hydroxylation activity is inactivated faster than catecholase activity Mucuna pruriens

Inhibitors

Inhibitors Comment Organism Structure
azide 1 mM, 60% inhibition of catecholase activity Mucuna pruriens
diethyldithiocarbamate 0.05 mM, 97% inhibition of tyrosinase activity, 83% inhibition of catecholase activity Mucuna pruriens
L-ascorbic acid
-
Mucuna pruriens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
tyramine
-
Mucuna pruriens
1.09
-
dopamine
-
Mucuna pruriens
1.45
-
Adrenaline bitartrate
-
Mucuna pruriens
1.8
-
L-tyrosine
-
Mucuna pruriens
2
-
4-[(1R)-2-amino-1-hydroxyethyl]benzene-1,2-diol
-
Mucuna pruriens
2.5
-
alpha-Methyl-dopa
-
Mucuna pruriens
5.55
-
D-Dopa
-
Mucuna pruriens
6.66
-
L-Dopa
-
Mucuna pruriens
8
-
N-Formyl-L-tyrosine
-
Mucuna pruriens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE Mucuna pruriens
90000
-
gel filtration Mucuna pruriens

Organism

Organism UniProt Comment Textmining
Mucuna pruriens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Celite chromatography, DEAE-Sepharose Mucuna pruriens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Mucuna pruriens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
658
-
L-dopa oxidation Mucuna pruriens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adrenaline bitartrate + O2
-
Mucuna pruriens ?
-
?
alpha-methyl-dopa + O2
-
Mucuna pruriens alpha-methyldopaquinone + H2O
-
?
D-dopa + O2
-
Mucuna pruriens dopaquinone + H2O
-
r
dopa + 1/2 O2 L-dopa Mucuna pruriens dopaquinone + H2O
-
?
dopa + 1/2 O2 D-dopa Mucuna pruriens dopaquinone + H2O
-
?
dopamine + 1/2 O2
-
Mucuna pruriens dopamine quinone + H2O
-
?
L-tyrosine + O2 + AH2
-
Mucuna pruriens L-dopa + H2O + A
-
?
N-formyl-L-tyrosine + O2
-
Mucuna pruriens ?
-
?
noradrenaline + O2
-
Mucuna pruriens ?
-
?
tyramine + O2
-
Mucuna pruriens 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Mucuna pruriens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 catecholase activity Mucuna pruriens
7.5
-
tyrosine hydroxylase Mucuna pruriens